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Merck
CN

G3293

Supelco

葡萄糖测定试剂

for use with enzymatic assay kits GAHK20, SCA20, FA20, SA20

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About This Item

UNSPSC代码:
12164500
NACRES:
NA.84

质量水平

分析物化学类别

sugars (glucose)

技术

photometry: suitable

杂质

≤0.005% Creatine phosphokinase, glutathione reductase, myokinase, NADH-oxidase,NADPH oxidase, phosphogluconic dehydrogenase, phosphoglucose isomerase, lactic dehydrogenase
<0.01% ATP-ase, myokinase

应用

food and beverages
general analytical

储存温度

2-8°C

特异性

与以下底物发生交叉反应可导致活性 < 0.05%:果糖、葡聚糖、景天庚糖、阿拉伯糖、半乳糖、木糖、海藻糖、甘露糖、山梨醇、+/-岩藻糖或核糖。

应用

加入包装所示体积(20mL 或 50mL)的去离子水。
通过己糖激酶-葡萄糖-6-磷酸的酶法对食物和其他物质中的葡萄糖进行定量测定。

相关产品

产品编号
说明
价格

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

常规特殊物品

从最新的版本中选择一种:

分析证书(COA)

Lot/Batch Number

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Is it possible to adapt Product G3293, Glucose Assay Reagent, for use with a 96 well plate reader?

    In principle, it should be possible to adapt assay procedures using this reagent to 96-well plate readers. Volumes of reagent, water, standard, and unknown should be reduced to fit the capacity of the test wells, and kept in the same proportions as those used with a spectrophotometer. The volume reduction will likely be 1:4 or 1:5. With the shorter "pathlength" of a multiwell plate, the sensitivity of the assay would likely decrease.

  4. Is magnesium required for Product G3293, Glucose Assay Reagent?

    Magnesium, an activator of hexokinase, is included in the assay reagent. There is no need to add a magnesium salt to the reagent before use.

  5. What is the detection limit of Product G3293, Glucose Assay Reagent?

    This reagent should be able to detect glucose down to approximately 0.01 mg/mL using a spectrophotometer with a 1 cm path length.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Anne Riemann et al.
PloS one, 6(7), e22445-e22445 (2011-08-06)
Tumor micromilieu often shows pronounced acidosis forcing cells to adapt their phenotype towards enhanced tumorigenesis induced by altered cellular signalling and transcriptional regulation. In the presents study mechanisms and potential consequences of the crosstalk between extra- and intracellular pH (pH(e)
Michele Bertazzini et al.
Frontiers in plant science, 7, 288-288 (2016-03-26)
Numerous angiosperms rely on pollinators to ensure efficient flower fertilization, offering a reward consisting of nourishing nectars produced by specialized floral cells, known as nectaries. Nectar components are believed to derive from phloem sap that is enzymatically processed and transformed
Mor Tsamir-Rimon et al.
The New phytologist, 229(3), 1398-1414 (2020-09-04)
Carbon reserve use is a major drought response in trees, enabling tree survival in conditions prohibiting photosynthesis. However, regulation of starch metabolism under drought at the whole-tree scale is still poorly understood. To this end, we combined measurements of nonstructural
Daniel S Straus
Molecular cancer, 12, 78-78 (2013-07-20)
Inflammation is a well-known etiological factor for colorectal cancer, but mechanisms underlying the linkage between inflammation and cancer are incompletely understood. We hypothesized that two pro-inflammatory cytokines, TNFα and IL-17, might play a role in promoting colorectal carcinogenesis. Aerobic glycolysis
Chao Zhang et al.
The Journal of animal ecology, 85(6), 1431-1441 (2016-10-26)
A key challenge for ecologists is to predict how single and joint effects of global warming and predation risk translate from the individual level up to ecosystem functions. Recently, stoichiometric theory linked these levels through changes in body stoichiometry, predicting

实验方案

Enzymatic Assay of α-Glucosidase by the Modified Boehenger Procedure (EC 3.2.1.20)

改良Boehenger法酶促检测α-葡萄糖苷酶(EC 3.2.1.20)

To standardize an enzymatic assay procedure of cellulase.

该实验方案使用分光光度法测定纤维素酶活性。黑曲霉纤维素酶催化内-1,4-β-D-糖苷键的水解。

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