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用途
sufficient for 100 colorimetric tests
检测方法
colorimetric
相关疾病
cancer; neurological disorders; hematological disorder
储存温度
−20°C
基因信息
human ... GCK(2645) , HK1(3098) , HK2(3099) , HK3(3101)
mouse ... GCK(103988) , HK1(15275) , HK2(15277) , HK3(212032)
rat ... GCK(24385) , HK1(25058) , HK2(25059) , HK3(25060)
一般描述
己糖激酶，催化醛酸和酮己糖的ATP 依赖性磷酸化为己糖-6-磷酸，催化许多重要生化途径的第一步。在哺乳动物组织中，葡萄糖是己糖激酶的主要底物。哺乳动物组织中有四种己糖激酶同工酶（HK-I、-II、-III和-Iv），它们的动力学和调节性质不同，以及组织分布和辅因子的使用不同。己糖激酶催化大多数相关葡萄糖代谢途径的第一步。己糖激酶活性的改变与多种疾病相关，例如X 连锁的肌营养不良症、溶血性贫血和癌症。
应用
己糖激酶比色检测试剂盒已用于测量己糖激酶的活性。
适用性
适用于测定己糖激酶活性，分析葡萄糖代谢和细胞信号，以及筛选血清、动物组织（肝脏、心脏、肾脏等）和细胞培养物（粘附或悬浮细胞）中的抗糖尿病药物。
原理
己糖激酶比色测定试剂盒提供了一种简单、直接的方法来测量各种样品中的己糖激酶活性。己糖激酶活性通过偶联酶测定法测定，其中葡萄糖通过己糖激酶转化为葡萄糖-6-磷酸，其被葡萄糖-6-磷酸脱氢酶氧化形成 NADH。所得到的NADH 减少无色探针，从而产生与己糖激酶活性成比例的比色（450nm）产物。一个单位的HK是在室温下，在pH 8.0 下，每分钟产生1.0 μmole NADH的酶的量。
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Hexokinase domain containing 1, a recently discovered putative fifth hexokinase, is hypothesized to play key roles in glucose metabolism. Specifically, during pregnancy in a recent genome wide association study (GWAS), a strong correlation between HKDC1 and 2-h plasma glucose in
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实验方案
Measure hexokinase activity using a continuous spectrophotometric rate-determination assay at 340 nm, catalyzing D-hexose sugar phosphorylation using ATP.
在测定己糖激酶活性时，使用了一种在340nm处的连续分光光度法进行测定。己糖激酶使用ATP作为磷酸的来源来催化D-己糖的磷酸化。
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