跳转至内容
Merck
CN
所有图片(2)

文件

G5080

Sigma-Aldrich

葡聚糖凝胶 ® G-50

Fine

登录查看公司和协议定价
所有图片(2)
别名:
Gel filtration resin, Sephadex G-50 Fine Medium
CAS号:
9048-71-9
UNSPSC代码:
23151817
NACRES:
NA.56

质量水平

200

技术

affinity chromatography: suitable

基质活性基团

phase

溶胀

1 g swells to 9-11 mL

珠子尺寸

20-80 μm

应用

life science and biopharma

相容性

Cytiva

相关类别

一般描述

Sephadex® G-50是一种用于亲和层析、蛋白质层析和凝胶过滤层析的凝胶过滤介质。Sephadex 是一种由葡聚糖和环氧氯丙烷交联而成的珠状凝胶。
分馏范围（MW）
球状蛋白质：1,500-30,000
葡聚糖：500-10,000
Sephadex G-50 Fine is a widely used gel filtration resin designed for desalting and buffer exchange of biomolecules with a molecular weight greater than 30,000. Its small bead size contributes to higher efficiency in these processes. Different types of Sephadex vary in terms of cross-linking, resulting in differences in swelling and molecular fractionation range. Sephadex G-50 is one of five G-types available, ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 comes in four different particle sizes, such as Coarse, Medium, Fine, and Superfine.

应用

Sephadex® G-50 适用于：

  • 低分子量和高分子量分子的分离
  • 亲和色谱法、蛋白质色谱法和凝胶过滤色谱法
Sephadex® G-50 has been used:
  • to remove the non-entrapped carboxyfluorescein (CF) from the liposome suspension
  • in the purification of monoclonal antibody (mAb) humanized MN-14 by centrifuged size-exclusion chromatography
  • in desalting the recombinant enzymes eluted in the fraction five and six

特点和优势

  • Sephadex 脱盐被认为优于透析，因为它节省时间，稀释系数低，即便使用少量样品也能恢复高活性
  • Desalts, removes contaminants, and transfers to a new buffer in one step.
  • Suitable for DNA purification from small molecules using gel filtration.
  • Features a small bead size, resulting in shorter diffusion distances.
  • Considered a classic gel filtration resin.

其他说明

G5080-100G′更新后的产品编号为 GE17-0042-01
G5080-500G′更新后的产品编号为 GE17-0042-02

法律信息

Sephadex is a registered trademark of Cytiva

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What does "G-50" mean in Sephadex®  G-50, Product G5080?

    "G" stands for gel.  "50" refers to the water regain of the gel, in this instance, 5.0 g water per g dry gel.

  4. What is the best buffer to use with Sephadex®  G-50, Product G5080?

    Sephadex® G-50 is very flexible in the buffers that can be used with it.  Essentially any common aqueous buffer can be used with Sephadex® G-50.  It is advised to filter buffers through a 0.22 micron filter before use.

  5. How stable is Sephadex® G-50, Product G5080, after swelling?

    Sephadex® is stable in water, salt solutions, organic and denaturing solvents.  If necessary, the gel can be removed from the column and sterilized by autoclaving at 120 deg C, pH 7. The pH stability is limited to low strengths and short times at extremes of pH 2-10, particularly in the acid range.  After any washes using solutions at extremes of acidic or basic pH, the Sephadex®  should be washed and reequilibrated with buffer at neutral pH as promptly as possible.

  6. How can I clean the Sephadex®  G-50, Product G5080, after use?

    To clean the gel, you can wash the gel with 2 column volumes (CV) of 0.2 M NaOH, outside of the column because the gel will swell in basic pH, or in a solution of non-ionic detergent.  Then you can rinse with water and re-equilibrate with 2-3 CV of buffer. If the resin is to be stored (longer than ~24 hours), equilibrate the resin with buffer containing an antimicrobial such as 20% ethanol or 0.02% sodium azide.

  7. What kind of separation can I expect from Sephadex®  G-50, Product G5080?

    As a general rule of thumb, Sephadex® G-50 is appropriate for the separation of molecules of ~30 kDa from molecules ~1.5 kDa

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Relaxation of tyrosine pathway regulation underlies the evolution of betalain pigmentation in Caryophyllales
Lopez-Nieves S, et al.
The New phytologist, 217, 896-908 (2018)
L Zhang et al.
The EMBO journal, 14(2), 313-320 (1995-01-16)
Heme is a prosthetic group for numerous enzymes, cytochromes and globins, and it binds tightly, sometimes covalently, to these proteins. Interestingly, heme also potentiates binding of the yeast transcriptional activator HAP1 to DNA and inhibits mitochondrial import of the mammalian
Clinical-scale radiolabeling of a humanized anticarcinoembryonic antigen monoclonal antibody, hMN-14, with residualizing 131I for use in radioimmunotherapy
Govindan SV, et al.
Journal of Nuclear Medicine, 46(1), 153-159 (2005)
Samuel Lopez-Nieves et al.
The New phytologist, 217(2), 896-908 (2017-10-11)
Diverse natural products are synthesized in plants by specialized metabolic enzymes, which are often lineage-specific and derived from gene duplication followed by functional divergence. However, little is known about the contribution of primary metabolism to the evolution of specialized metabolic
Jennifer E Dawson et al.
Nature communications, 11(1), 3146-3146 (2020-06-21)
Phosphorylation of intrinsically disordered eIF4E binding proteins (4E-BPs) regulates cap-dependent translation by weakening their ability to compete with eIF4G for eIF4E binding within the translation initiation complex. We previously showed that phosphorylation of T37 and T46 in 4E-BP2 induces folding
查看所有相关文献

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门