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G100120

Sigma-Aldrich

葡聚糖凝胶 ® G-100

Medium

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CAS号:
9050-94-6
MDL编号:
MFCD00132235
UNSPSC代码:
23151817
NACRES:
NA.56

质量水平

100

形式

slurry

技术

LPLC: suitable

基质

Agarose

基质活性基团

polymer

溶胀

1 g swells to 15-20 mL gel

珠子尺寸

40-120 μm

分离技术

size exclusion (SEC)

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相关类别

一般描述

Sephadex®葡聚糖凝胶由α糖苷键连接的葡萄糖重复单元组成，这种结构可确保更多的适配体结合位点和更大的结合容量。它适用于大规模纯化，性能稳定并可多次使用。
分级范围(MW)
球状蛋白：4,000 - 150,000
右旋糖酐：1,000-100,000

应用

Sephadex®葡聚糖凝胶G-100是一种凝胶过滤介质，用于蛋白质色谱、亲和色谱和凝胶过滤色谱。
它已用于：
  • 通过凝胶过滤色谱柱纯化α-淀粉酶
  • 通过凝胶过滤色谱柱纯化从粗背鳐皮肤中提取的多糖组分
  • 通过体积排阻去除交联氧化铁(CLIO)-精蛋白连接(B)肽中多余的精蛋白

其他说明

G100120-100G的新货号为GE17-0060-01

法律信息

Sephadex is a registered trademark of Cytiva

替代产品

产品编号
说明
价格

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. My Sephadex® G-100 column is cracking. What is the maximum operating pressure that Product G100120 can take?

    The maximum operating pressure for this gel is 96 cm water = 1.6 psi.

  4. What is the maximum flow rate for Product G100120, Sephadex® G-100 columns?

    The maximum flow rate is 4.2 ml/min. Data are based on columns of 2.5 cm diameter with a bed height of 30 cm.

  5. How do I determine which size to use for Product G100120, Sephadex® G-100 columns?

    Generally, larger particles offer faster flow rates than finer particles, but poorer resolution.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Mohamed Ben Mansour et al.
Thrombosis research, 123(4), 671-678 (2008-07-12)
The polysaccharide fraction from the skin of the ray Raja radula was extracted, characterized and assayed for anticoagulant activity. A whole polysaccharidic fraction was extracted from the skin of the ray Raja radula by papain digestion followed by cetylpyridinium chloride
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Berner, N.J., and Ingermann, R.L.
The Journal of Experimental Biology, 140, 147-153 (1988)
R H Coleman et al.
Current microbiology, 32(3), 147-150 (1996-03-01)
Pseudomonas syringae isolate BR2R produces tabtoxin, a beta-lactam-containing antibiotic, and the causative agent of wildfire disease of green bean (Phaseolus vulgaris). beta-Lactamase production has been suggested as the mechanism that protects P. syringae from tabtoxin. We sought to determine whether
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