质量水平
技术
buffer exchange: suitable
基质活性基团
phase
溶胀
1 g swells to 12-15 mL gel
珠子直径
40-120 μm
应用
life science and biopharma
相容性
Cytiva
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一般描述
Sephadex G-75葡聚糖凝胶广泛用于脱盐和缓冲液置换。这种凝胶过滤树脂由葡聚糖与环氧氯丙烷交联而成。Sephadex产品有各种不同的交联度,因此其溶胀程度和分子分离范围也不同。
Sephadex® G-75是凝胶过滤介质,用于凝胶过滤色谱和蛋白质色谱。Sephadex® G-75已用于研究哺乳动物大脑的胱抑素,开发新型口服或其他抗感染药物的候选药物,并从响尾蛇毒液中纯化促凝金属蛋白酶。
应用
Sephadex® G-75葡聚糖凝胶可用于凝胶过滤色谱分离胃蛋白酶消化物中的产物,也用于凝胶过滤法脱盐低密度脂蛋白(LDL)。
葡聚糖® G-75用于通过凝胶过滤对低密度脂蛋白(LDL)进行纯化。
特点和优势
- 一步完成快速脱盐、除污染和置换新缓冲液。
- 适合分子量超过80,000的生物大分子的缓冲液置换和纯化。
- 具有Sephadex G型的最高排阻极限80,000。
- 经典的凝胶过滤树脂。
法律信息
Sephadex is a registered trademark of Cytiva
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
Integrin alpha IIb beta 3 (platelet GPIIb-IIIa) recognizes multiple sites in fibronectin.
Journal of biological and chemical chronicles, 266(34), 23323-23328 (1991)
mBio, 12(4), e0136821-e0136821 (2021-08-04)
The human gut microbiota (HGM) contributes to the physiology and health of its host. The health benefits provided by dietary manipulation of the HGM require knowledge of how glycans, the major nutrients available to this ecosystem, are metabolized. Arabinogalactan proteins
Rapid purification of fluorescent dye-labeled products in a 96-well format for high-throughput automated DNA sequencing.
Nucleic acids research, 23(23), 4930-4931 (1995-12-11)
Inhibition of cyclic AMP-and cyclic GMP-mediated dilations in isolated arteries by oxidized low density lipoproteins.
Arteriosclerosis and Thrombosis, 12(2), 180-186 (1992)
Indian journal of experimental biology, 49(5), 366-374 (2011-05-28)
Antimicrobial activity of protease inhibitor isolated from Coccinia grandis (L.) Voigt. has been reported. A 14.3 kDa protease inhibitor (PI) was isolated and purified to homogeneity by ammonium sulfate precipitation (20-85% saturation), sephadex G-75, DEAE sepharose column and trypsin-sepharose affinity
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