产品名称
Sephadex® G-25, Fine
SMILES string
O1[C@H]([C@@H]([C@@H]([C@@H]([C@H]1CO)O)O)O)O.OC(CO)CO
InChI
1S/C6H12O6.C3H8O3/c7-1-2-3(8)4(9)5(10)6(11)12-2;4-1-3(6)2-5/h2-11H,1H2;3-6H,1-2H2/t2-,3-,4-,5-,6-;/m1./s1
InChI key
BCPVZFFJGCURNR-OCOFDJSDSA-N
technique(s)
buffer exchange: suitable
matrix active group
phase
swelling
1 g swells to 4-6 mL
bead size
20-80 μm
application(s)
life science and biopharma
compatibility
Cytiva
Quality Level
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Application
Sephadex® G-25 用于尺寸排阻层析，去除标记蛋白中的多余染料。
General description
Sephadex®是珠状凝胶过滤介质，由葡聚糖与环氧氯丙烷在碱性条件下交联制备而成。 用于亲和层析、蛋白层析和凝胶过滤层析。
分级分离范围（MW）
球状蛋白：1,000 - 5,000
右旋糖苷：100 - 5,000
分级分离范围（MW）
球状蛋白：1,000 - 5,000
右旋糖苷：100 - 5,000
Other Notes
G10050-100G′更新的产品编号为GE17-0032-01
G10050-500G′更新的产品编号为GE17-0032-02
G10050-500G′更新的产品编号为GE17-0032-02
Legal Information
Sephadex is a registered trademark of Cytiva
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Celine Hoffmann et al.
Analytical biochemistry, 372(1), 62-71 (2007-09-11)
Fluorescein isothiocyanate (FITC) is a well-known probe for labeling biologically relevant proteins. However, the impact of the labeling procedure on protein structure and biological activities remains unclear. In this work, FITC-labeled human plasma fibronectin (Fn) was developed to gain insight
Jennifer Belyea et al.
Biochemistry, 45(48), 14275-14284 (2006-11-30)
The study of axial ligation by anionic ligands to ferric heme iron by resonance Raman spectroscopy provides a basis for comparison of the intrinsic electron donor ability of the proximal histidine in horse heart myoglobin (HHMb), dehaloperoxidase (DHP), and horseradish
Conformations of a metastable SH3 domain characterized by smFRET and an excluded-volume polymer model.
Mazouchi A, et al.
Biophysical Journal, 110(7), 1510-1522 (2016)
Jana Schwarzer et al.
Electrophoresis, 29(20), 4203-4214 (2008-10-18)
Glycoproteins, such as monoclonal antibodies as well as recombinant and viral proteins produced in mammalian cell culture play an important role in manufacturing of many biopharmaceuticals. To ensure consisting quality of the corresponding products, glycosylation profiles have to be tightly
P M G Paiva et al.
Bioresource technology, 88(1), 75-79 (2003-02-08)
A highly purified trypsin inhibitor was obtained from Echinodorus paniculatus when an extract prepared from E. paniculatus seed flour (25 gl(-1), with 0.1 M ammonium acetate buffer, pH 8.3, under agitation for 6 min at 28 degrees C) was chromatographed
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