SMILES string
O1[C@H]([C@@H]([C@@H]([C@@H]([C@H]1CO)O)O)O)O.OC(CO)CO
InChI
1S/C6H12O6.C3H8O3/c7-1-2-3(8)4(9)5(10)6(11)12-2;4-1-3(6)2-5/h2-11H,1H2;3-6H,1-2H2/t2-,3-,4-,5-,6-;/m1./s1
InChI key
BCPVZFFJGCURNR-OCOFDJSDSA-N
technique(s)
buffer exchange: suitable
matrix active group
phase
swelling
1 g swells to 4-6 mL
bead size
50-150 μm
application(s)
life science and biopharma
compatibility
Cytiva
Quality Level
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General description
Sephadex® G-25葡聚糖凝胶由葡聚糖与环氧氯丙烷交联而成，用作体积排阻和凝胶过滤树脂。不同类型的Sephadex的交联度不同，因此其溶胀程度和分子分离范围也不同。Sephadex G-25是G型Sephadex中的一种型号。G型系列分5种型号，具有不同的分子量分离范围。其中，G-10适合分离小分子，G-75适合大分子。Sephadex G-25保证可靠供应并提供全面的技术和监管支持，以满足工业要求。
Application
Sephadex® G-25葡聚糖凝胶可用于通过凝胶渗透色谱将标记蛋白与未反应染料N-羟基琥珀酰亚胺酯(NHS)分离。
分馏范围（MW）
右旋糖酐：100 - 5,000
球状蛋白质1,000 - 5,000
Sephadex® G-25已用于：
右旋糖酐：100 - 5,000
球状蛋白质1,000 - 5,000
Sephadex® G-25已用于：
- 蛋白酶脱盐
- 在放射性碘标记oTP-1的表征和受体分析确认过程中，用色谱柱从游离碘中分离放射性碘标记的oTP-1
Biochem/physiol Actions
Sephadex® G-25葡聚糖凝胶是应用广泛的凝胶过滤树脂，主要用于工业应用中的脱盐和缓冲液置换。亲水基质可最大限度减少非特异性吸附，获得高收率的蛋白和核酸。Sephadex® G-25常用于亲和层析、蛋白质层析和凝胶过滤层析。
Features and Benefits
- 一步完成快速脱盐、除污染和置换新缓冲液。
- 高回收率，极低的样品稀释度。
- 提供预装树脂的HiPrep脱盐柱和HiTrap脱盐柱，用于方便快速地脱盐
- 专门设计用作生物工艺树脂，适合工业应用。
Legal Information
Sephadex is a registered trademark of Cytiva
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Characterization of endometrial receptors for ovine trophoblast protein-1 during the estrous cycle and early pregnancy in sheep
Knickerbocker JJ and Niswender GD
Biology of Reproduction, 40(2), 361-369 (1989)
P R Howard et al.
Clinical chemistry, 34(2), 324-330 (1988-02-01)
A monoclonal-antibody-based competitive radioimmunoassay for measuring human protein C is reported. With use of a purified protein C standard, the solid-phase assay was sensitive to less than 80 micrograms of protein C per liter. Intraassay CVs ranged from 5% to
David R Halpin et al.
PLoS biology, 2(7), E173-E173 (2004-06-29)
Recently reported technologies for DNA-directed organic synthesis and for DNA computing rely on routing DNA populations through complex networks. The reduction of these ideas to practice has been limited by a lack of practical experimental tools. Here we describe a
J J Knickerbocker et al.
Biology of reproduction, 40(2), 361-369 (1989-02-01)
Scatchard analysis was used to determine the distribution, number, and affinity of unoccupied receptors for ovine trophoblast protein-1 (oTP-1) in endometrium of sheep throughout the estrous cycle and early pregnancy. In Experiment I, oTP-1 receptor characteristics were determined in membrane
K M Yocom et al.
Proceedings of the National Academy of Sciences of the United States of America, 79(22), 7052-7055 (1982-11-01)
A stable complex between pentaammineruthenium(III) and histidine-33 in horse heart ferricytochrome c is formed in the reaction between aquopentaammineruthenium(II) and the protein at pH 7. HPLC of the tryptic hydrolysate of the modified protein was employed to identify the pentaammineruthenium
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