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FLASC

Sigma-Aldrich

腺苷 5′-三磷酸 (ATP) 生物发光体细胞检测试剂盒

for cellular ATP determination

别名:

ATP bioluminescence assay kit

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About This Item

UNSPSC代码:
12352202
eCl@ss:
32160414
NACRES:
NA.26

质量水平

200

储存温度

−20°C

相关类别

一般描述

腺苷5′-三磷酸(ATP)生物发光体细胞检测试剂盒用于通过生物发光法测定活性体细胞悬液中释放的腺苷-5′-三磷酸(ATP)。若假定每个活细胞中的ATP含量保持不变，即可推算出细胞浓度。只选择计算活性体细胞数目是因为死亡后细胞中的ATP水平会快速下降。

应用

腺苷5′-三磷酸(ATP)生物发光体细胞检测试剂盒用于准确测定从100多名人类测试患者的血液中分离的嗜中性粒细胞中存在的ATP水平。

仅试剂盒组分

产品编号
说明
  • FL-AAB Dilution buffer 1 mL/vial
  • FL-AAM Assay mix 1 mL/vial
  • FL-AAS ATP standard 1 mL/vial
  • FL-SAR Somatic cell releasing reagent 1 mL/vial

象形图

CorrosionEnvironment
GHS05,GHS09

警示用语：

Danger

危险声明

H318,H410

危险分类

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1

储存分类代码

10 - Combustible liquids

法规信息

含少量动物源组分生物产品

从最新的版本中选择一种：

分析证书(COA)

Lot/Batch Number
SLCP4458
SLCJ9482
SLCH0845
SLCC0450
SLCC0453

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Can lysates be stored in the Somatic Cell ATP Releasing Reagent of Adenosine 5´-triphosphate (ATP) bioluminescent somatic cell assay kit, Product FLASC?

    In general, no. The working Somatic Cell ATP Releasing Reagent is intended for immediate use, and not for storing ATP in.  We have no specific information as to how long ATP will be stable in it.

  6. Can Adenosine 5´-triphosphate (ATP) bioluminescent somatic cell assay kit, Product FLASC, be used for adherent cells?

    We do not have a separate protocol for adherent cells. However, the somatic cell lysis reagent should be able to lyse cells that are adherent.  The reagent contains enough detergent that the cells will be lysed. Once cells are lysed, it is best to assay the sample immediately.

  7. What is the optimal dilution for tissue extracts when using Adenosine 5´-triphosphate (ATP) bioluminescent somatic cell assay kit, Product FLASC?

    Sigma-Aldrich has not tested this product with tissue extracts. You will need to perform trials to determine optimal dilution of the sample.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Y Zhao et al.
Redox biology, 12, 129-138 (2017-02-24)
The signaling of reactive oxygen species (ROS) is essential for the maintenance of normal cellular function. However, whether and how ROS regulate stem cells are unclear. Here, we demonstrate that, in transgenic mice expressing the human manganese superoxide dismutase (MnSOD)
Linqing Zhen et al.
Reproductive toxicology (Elmsford, N.Y.), 59, 66-79 (2015-11-20)
Hexavalent chromium reportedly induces reproductive toxicity and further inhibits male fertility in mammals. In this study, we investigated the molecular mechanism by which hexavalent chromium affects motility signaling in boar spermatozoa in vitro. The results indicated that Cr(VI) decreased sperm
Sarah Myhill et al.
International journal of clinical and experimental medicine, 2(1), 1-16 (2009-05-14)
This study aims to improve the health of patients suffering from chronic fatigue syndrome (CFS) by interventions based on the biochemistry of the illness, specifically the function of mitochondria in producing ATP (adenosine triphosphate), the energy currency for all body
Li Xinhong et al.
Theriogenology, 116, 71-82 (2018-05-21)
The reproductive efficiency of Meishan pigs is higher than that of Duroc pigs, but the underlying molecular mechanism for this disparity remains unclear. No systematic quantitative proteomics studies, comparing global proteins in Meishan and Duroc boar spermatozoa have been reported.
Amanda do Rocio Andrade Pires et al.
Carbohydrate polymers, 170, 33-42 (2017-05-20)
A mannogalactoglucan (RK2-Ab; M
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