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FLAA

Sigma-Aldrich

腺苷 5′-三磷酸(ATP)生物发光测定试剂盒

for ATP quantitation

别名:

ATP Bioluminescence Assay, ATP Determination Kit, Luminescent ATP Detection kit

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选择尺寸

1 KIT
¥5,251.95

¥5,251.95


国内现货,预计发货时间2025年4月17日详情


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变更视图
1 KIT
¥5,251.95

About This Item

EC 号:
UNSPSC代码:
12352202
eCl@ss:
32160414
NACRES:
NA.26

¥5,251.95


国内现货,预计发货时间2025年4月17日详情


获取大包装报价

储存温度

−20°C

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一般描述

5′- 三磷酸腺苷 (ATP) 生物发光测试剂盒用于样品中 ATP 的定量荧光测定。当荧光素酶催化 D- 荧光素的氧化时,ATP 被消耗并发光。当 ATP 为限制性试剂时,发出的光与样品中存在的 ATP 成正比。

应用

5′-三磷酸腺苷 (ATP) 生物发光测试剂盒还用于定量分析人神经元细胞 3D 矩阵、[1]缺血肝细胞、[2]各种细菌培养液[3][4]和溶酶体中的 ATP。[5]

仅试剂盒组分

产品编号
说明

  • FL-AAB Dilution buffer 1 mL/vial

  • FL-AAM Assay mix 1 mL/vial

  • FL-AAS ATP standard 1 mL/vial

象形图

Corrosion

警示用语:

Danger

危险声明

预防措施声明

危险分类

Eye Dam. 1

储存分类代码

10 - Combustible liquids

法规信息

含少量动物源组分生物产品

  • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number

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    1. How many reactions can one do with Product FLAA, ATP Bioluminescent Assay Kit (one vial of FLAAM)?

      When one reconstitutes the vial to 5 ml, as directed, one can perform 50 assays using 100 ul per test. If one dilutes 1:5 one can get 250 tests per kit.

    2. What is the sensitivity of Product FLAA, ATP Bioluminescent Assay Kit the assay mix?

      With FLAAM diluted 1:5, one can easily measure 1 pmol ATP in 100 μl (10 nM) with 50 μl of the reagent.

    3. What  is the stability of the Product FLAA, ATP Bioluminescent Assay Kit ATP stock solution?

      The ATP standard stock solution (FLAAS) is stable for at least 24 hours at 0-5°C, or for over two weeks if stored frozen at -20°C.

    4. Can Product FLAA, ATP Bioluminescent Assay Kit assay mix be used on cells and tissues?

      This reagent measures only soluble ATP. Cells must be washed and then lysed to obtain a soluble ATP extract. Tissues must be homogenized and clarified to provide an extract. The supernatant solution can then be used for assay.

    5. How can one check for inhibitors in the samples when using Product FLAA, ATP Bioluminescent Assay Kit?

      One must add an amount of ATP from a standard solution equivalent to the ATP already found in the sample. If the signal is doubled, the absence of an inhibitor is indicated.

    6. Which document(s) contains shelf-life or expiration date information for a given product?

      If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

    7. How do I get lot-specific information or a Certificate of Analysis?

      The lot specific COA document can be found by entering the lot number above under the "Documents" section.

    8. How do I find price and availability?

      There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

    9. What is the Department of Transportation shipping information for this product?

      Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

    10. My question is not addressed here, how can I contact Technical Service for assistance?

      Ask a Scientist here.

    John F C Steele et al.
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    The Journal of physiology, 594(15), 4253-4266 (2016-08-02)
    SLC17A9 proteins function as a lysosomal ATP transporter responsible for lysosomal ATP accumulation. P2X4 receptors act as lysosomal ion channels activated by luminal ATP. SLC17A9-mediated ATP transport across the lysosomal membrane is suppressed by Bafilomycin A1, the V-ATPase inhibitor. SLC17A9
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    Journal of Food Research, 2(4), 37-37 (2013)
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    The combination of hypothermic and normothermic machine perfusion (HMP+NMP) of the liver provides individual benefits of both techniques, improving the rescue of marginal organs. The aim of this study was to investigate the effect on the bioenergetic status and the

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