跳转至内容
Merck
CN
所有图片(1)

主要文件

查看所有文档

安全信息

FLAA

Sigma-Aldrich

腺苷 5′-三磷酸（ATP）生物发光测定试剂盒

for ATP quantitation

别名:

ATP Bioluminescence Assay, ATP Determination Kit, Luminescent ATP Detection kit

登录查看公司和协议定价
所有图片(1)

About This Item

EC 号:
213-579-1
UNSPSC代码:
12352202
eCl@ss:
32160414
NACRES:
NA.26

质量水平

200

储存温度

−20°C

相关类别

一般描述

5′- 三磷酸腺苷 (ATP) 生物发光测试剂盒用于样品中 ATP 的定量荧光测定。当荧光素酶催化 D- 荧光素的氧化时，ATP 被消耗并发光。当 ATP 为限制性试剂时，发出的光与样品中存在的 ATP 成正比。

应用

5′-三磷酸腺苷 (ATP) 生物发光测试剂盒还用于定量分析人神经元细胞 3D 矩阵、缺血肝细胞、各种细菌培养液和溶酶体中的 ATP。

仅试剂盒组分

产品编号
说明
  • FL-AAB Dilution buffer 1 mL/vial
  • FL-AAM Assay mix 1 mL/vial
  • FL-AAS ATP standard 1 mL/vial

象形图

Corrosion
GHS05

警示用语：

Danger

危险声明

H318

预防措施声明

P280 - P305 + P351 + P338

危险分类

Eye Dam. 1

储存分类代码

10 - Combustible liquids

法规信息

含少量动物源组分生物产品
常规特殊物品

从最新的版本中选择一种：

分析证书(COA)

Lot/Batch Number
SLCQ0442
SLCQ0441
SLCP6942
SLCM0389
SLCL1457

没有发现合适的版本？

如果您需要特殊版本，可通过批号或批次号查找具体证书。

搜索COA

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

  1. How many reactions can one do with Product FLAA, ATP Bioluminescent Assay Kit (one vial of FLAAM)?

    When one reconstitutes the vial to 5 ml, as directed, one can perform 50 assays using 100 ul per test. If one dilutes 1:5 one can get 250 tests per kit.

  2. What is the sensitivity of Product FLAA, ATP Bioluminescent Assay Kit the assay mix?

    With FLAAM diluted 1:5, one can easily measure 1 pmol ATP in 100 μl (10 nM) with 50 μl of the reagent.

  3. What  is the stability of the Product FLAA, ATP Bioluminescent Assay Kit ATP stock solution?

    The ATP standard stock solution (FLAAS) is stable for at least 24 hours at 0-5°C, or for over two weeks if stored frozen at -20°C.

  4. Can Product FLAA, ATP Bioluminescent Assay Kit assay mix be used on cells and tissues?

    This reagent measures only soluble ATP. Cells must be washed and then lysed to obtain a soluble ATP extract. Tissues must be homogenized and clarified to provide an extract. The supernatant solution can then be used for assay.

  5. How can one check for inhibitors in the samples when using Product FLAA, ATP Bioluminescent Assay Kit?

    One must add an amount of ATP from a standard solution equivalent to the ATP already found in the sample. If the signal is doubled, the absence of an inhibitor is indicated.

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

F Chen et al.
Journal of clinical microbiology, 32(11), 2791-2800 (1994-11-01)
A bioluminescent assay which employs the luciferin-luciferase ATP-dependent reaction was used to evaluate the viability of populations of Pneumocystis carinii derived from infected rat lungs. Contamination with host cells was reduced by a purification method which involved a combination of
Nadeshda Andreeva et al.
Applied microbiology and biotechnology, 102(12), 5235-5244 (2018-04-24)
Inorganic polyphosphate (polyP) is a significant regulatory and metabolic compound in yeast cells. We compared polyP content and localization, polyphosphatase activities, and transcriptional profile of polyP-related genes in industrially important methylotrophic yeasts, Hansenula polymorpha and Pichia pastoris. The increased need
Yuri L Boteon et al.
PloS one, 14(10), e0224066-e0224066 (2019-10-24)
The combination of hypothermic and normothermic machine perfusion (HMP+NMP) of the liver provides individual benefits of both techniques, improving the rescue of marginal organs. The aim of this study was to investigate the effect on the bioenergetic status and the
Hynek Mergental et al.
Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society, 24(10), 1453-1469 (2018-10-26)
Increased use of high-risk allografts is critical to meet the demand for liver transplantation. We aimed to identify criteria predicting viability of organs, currently declined for clinical transplantation, using functional assessment during normothermic machine perfusion (NMP). Twelve discarded human livers
Marco A Tavares et al.
Environmental toxicology and pharmacology, 40(1), 206-214 (2015-07-06)
Fipronil is an insecticide extensively used to control pests in crops and animals. There are relates of poisoning due to exposure of fipronil in mammals and the liver has been suggested as potential target. In this study, we evaluated the
查看所有相关文献

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门