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用途
sufficient for 100 tests (bioluminescent)
应用
pharmaceutical
检测方法
chemiluminescent
相关疾病
cancer
储存温度
−20°C
1 of 4
此商品 | OGS1119 | OGS1158 | OGS1157 |
|---|---|---|---|
| peptide tag location C-terminal | peptide tag location N-terminal | peptide tag location C-terminal | peptide tag location N-terminal |
| peptide cleavage thrombin | peptide cleavage TEV | peptide cleavage EKT | peptide cleavage EKT |
| origin of replication pUC (500 copies) | origin of replication pUC (500 copies) | origin of replication pUC (500 copies) | origin of replication pUC (500 copies) |
| mammalian cells selection puromycin | mammalian cells selection puromycin | mammalian cells selection puromycin | mammalian cells selection puromycin |
| promoter Promoter name: CMV | promoter Promoter name: CMV | promoter Promoter name: CMV | promoter Promoter name: CMV |
一般描述
ADP/ATP比例变化已用于区分细胞死亡和活率模式。ATP水平升高、ADP水平降低表示细胞正在增殖。相反，ATP降低、ADP的升高表示细胞凋亡或坏死，且相对于凋亡，坏死细胞ATP降低和ADP升高程度更为明显。
应用
- LOC554202 contributes to chordoma progression by sponging miR-377-3p and up-regulating SMAD3.：本文研究LOC554202通过miR-377-3p和SMAD3调节促进脊索瘤演进的分子机制。ADP/ATP比值检测试剂盒用于评估这些分子变化对细胞活性和增殖的影响(Xu et al., 2023)。
- FOXG1 improves mitochondrial function and promotes the progression of nasopharyngeal carcinoma.：本文研究FOXG1如何加强线粒体功能从而促进鼻咽癌进展。ADP/ATP比率检测试剂盒用于检测线粒体功能和能量代谢变化，解析癌细胞的生物能量特征(Xi et al., 2021)。
特点和优势
兼容高通量的操作系统。
适用性
适于检测细胞中的凋亡和坏死情况，或研究化合物对细胞增殖的影响。
原理
ADP/ATP比率测定试剂盒提供了一种简单直接的细胞ADP和ATP水平测定方法，可用于筛查细胞凋亡、坏死和增殖。该测定包括两个步骤。第一步，利用工作试剂裂解细胞以释放ATP和ADP。在存在萤光素酶的条件下，ATP立即与底物D-萤光素反应产生荧光。光强度是细胞内ATP浓度的直接指标。
萤光素酶
ATP + D-萤光素 + O2 ----------> 氧化萤光素 + AMP + PPi + CO2 + 荧光
第二步，ADP通过酶解反应转化为ATP。然后像第一步一样，新形成的ATP与D-荧光素反应。测得的第二个光强度代表样品中的总ADP和ATP浓度。
萤光素酶
ATP + D-萤光素 + O2 ----------> 氧化萤光素 + AMP + PPi + CO2 + 荧光
第二步，ADP通过酶解反应转化为ATP。然后像第一步一样，新形成的ATP与D-荧光素反应。测得的第二个光强度代表样品中的总ADP和ATP浓度。
相关产品
警示用语：
Warning
危险声明
危险分类
Aquatic Chronic 3 - Skin Sens. 1
储存分类代码
10 - Combustible liquids
法规信息
含少量动物源组分生物产品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Sangiliyandi Gurunathan et al.
Nanomaterials (Basel, Switzerland), 8(6) (2018-06-06)
The unique properties of gold nanoparticles (AuNPs) have attracted much interest for a range of applications, including biomedical applications in the cosmetic industry. The current study assessed the anti-oxidative effect of AuNPs against retinoic acid (RA)-induced loss of cell viability;
Aline G Cozer et al.
Lipids, 51(11), 1303-1307 (2016-10-25)
The present work assesses in vitro the role of human Stanniocalcin 1 (hSTC-1) in glucose metabolism in white retroperitoneal adipose tissue (WRAT) from fed rat. In the fed state, hSTC1 increases the incorporation of
Nidal Zeineh et al.
Cells, 8(7) (2019-07-13)
The 18 kDa translocator protein (TSPO) is an initiator of the mitochondrial apoptosis cascade. Cigarette smoke (CS) exposure provokes alterations in TSPO expression as well as upregulation of its related functions such as mitochondrial membrane potential (ΔψM) and reactive oxygen
Michael St Paul et al.
Cancer immunology research, 8(3), 321-333 (2020-01-23)
CD8+ T cells can be polarized into several different subsets as defined by the cytokines they produce and the transcription factors that govern their differentiation. Here, we identified the polarizing conditions to induce an IL22-producing CD8+ Tc22 subset, which is
Jian Cui et al.
BMC cancer, 19(1), 103-103 (2019-01-27)
In this report we examine candidate pathways perturbed by Compound Kushen Injection (CKI), a Traditional Chinese Medicine (TCM) that we have previously shown to alter the gene expression patterns of multiple pathways and induce apoptosis in cancer cells. We have
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