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E6654

Millipore

EZview™ 红色c-Myc亲和凝胶抗体

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别名:
Anti-c-Myc affinity resin, Anti-c-Myc agarose beads
UNSPSC代码:
12352200
NACRES:
NA.56

保质期

1 yr at 2‑8 °C

质量水平

200

分析物化学类别

proteins (c-Myc)

技术

affinity chromatography: suitable
immunoprecipitation (IP): suitable

容量

~0.05 mg/mL binding capacity

储存温度

2-8°C

相关类别

一般描述

EZ view 红色c-Myc亲和凝胶抗体是一种树脂，由抗c-Myc兔抗与4％琼脂糖珠共价结合而形成。亲和凝胶用于将c-Myc融合蛋白与样品（如细胞裂解物和组织）结合，以便在免疫沉淀测定准备过程中纯化c-Myc标签蛋白。红色染料可增强能见度，从而获得更有效的结果。
琼脂糖珠与大肠杆菌（E. coli）和哺乳动物细胞的N末端、Met-N末端和C末端c-Myc融合蛋白结合。

应用

EZview® 红色c-Myc亲和凝胶抗体适用于：
  • 类泛素化修饰检测
  • 对大肠杆菌（E. coli）或哺乳动物细胞的N-或C-末端c-Myc标签融合蛋白的亲和捕获（免疫沉淀）。
在小规模亲和捕获中，如免疫沉淀，难以在微量离心管中看到亲和基质。 在移液时意外吸取树脂，会导致结果产生数量差异。EZview™ 红色亲和凝胶可大大降低沉淀损失的风险。 EZview树脂的性能与传统无色亲和凝胶相同，但更易于区分沉淀和上清液。 这将获得更准确的数据，因为蛋白质损失减少。

特点和优势

  • 提高能见度 - 红色指示可降低意外吸取沉淀的风险
  • 通过减少意外损失，提高目标蛋白质的回收率
  • 更高的重现性 - 更稳定的得率

其他说明

亲和凝胶含有与琼脂糖珠共价结合的亲和纯化c-Myc兔多克隆抗体。 c-Myc标签对应于人c-Myc蛋白的氨基酸残基410-419（EQKLISEEDL）。

外形

溶剂为PBS的1:1(v/v)悬浮液，含有50％甘油和0.0015％Kathon CG/IPCII作为抗菌防腐剂

法律信息

EZview is a trademark of Sigma-Aldrich Co. LLC
SabreLite is a registered trademark of Pelican Products, Inc.

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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