¥3,321.49
预计发货时间2025年7月01日详情
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保质期
1 yr at 2‑8 °C
质量水平
分析物化学类别
proteins (c-Myc)
技术
affinity chromatography: suitable
immunoprecipitation (IP): suitable
容量
~0.05 mg/mL binding capacity
储存温度
2-8°C
1 of 4
此商品 | GF39754660 | GF50752212 | GF44676265 |
|---|---|---|---|
| form foil | form foil | form foil | form foil |
| assay 99.99% | assay 99.99% | assay 99.99% | assay 99.99% |
| bp 2750 °C (lit.) | bp 2750 °C (lit.) | bp 2750 °C (lit.) | bp 2750 °C (lit.) |
| mp 1535 °C (lit.) | mp 1535 °C (lit.) | mp 1535 °C (lit.) | mp 1535 °C (lit.) |
| density 7.86 g/mL at 25 °C (lit.) | density 7.86 g/mL at 25 °C (lit.) | density 7.86 g/mL at 25 °C (lit.) | density 7.86 g/mL at 25 °C (lit.) |
一般描述
EZ view 红色c-Myc亲和凝胶抗体是一种树脂，由抗c-Myc兔抗与4％琼脂糖珠共价结合而形成。亲和凝胶用于将c-Myc融合蛋白与样品（如细胞裂解物和组织）结合，以便在免疫沉淀测定准备过程中纯化c-Myc标签蛋白。红色染料可增强能见度，从而获得更有效的结果。
琼脂糖珠与大肠杆菌（E. coli）和哺乳动物细胞的N末端、Met-N末端和C末端c-Myc融合蛋白结合。
琼脂糖珠与大肠杆菌（E. coli）和哺乳动物细胞的N末端、Met-N末端和C末端c-Myc融合蛋白结合。
应用
EZview™红色c-Myc抗体亲和凝胶适于：
- SUMO化实验[1]
- 亲和捕获（免疫沉淀）来自大肠杆菌或哺乳动物细胞的N端或C端c-Myc标记融合蛋白。
在小规模亲和捕获中，如免疫沉淀，难以在微量离心管中看到亲和基质。 在移液时意外吸取树脂，会导致结果产生数量差异。EZview™ 红色亲和凝胶可大大降低沉淀损失的风险。 EZview树脂的性能与传统无色亲和凝胶相同，但更易于区分沉淀和上清液。 这将获得更准确的数据，因为蛋白质损失减少。
特点和优势
- 提高能见度 - 红色指示可降低意外吸取沉淀的风险
- 通过减少意外损失，提高目标蛋白质的回收率
- 更高的重现性 - 更稳定的得率
其他说明
亲和凝胶含有与琼脂糖珠共价结合的亲和纯化c-Myc兔多克隆抗体。 c-Myc标签对应于人c-Myc蛋白的氨基酸残基410-419（EQKLISEEDL）。
外形
溶剂为PBS的1:1(v/v)悬浮液，含有50％甘油和0.0015％Kathon CG/IPCII作为抗菌防腐剂
法律信息
EZview is a trademark of Sigma-Aldrich Co. LLC
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Keiko Sato et al.
Microbiology (Reading, England), 155(Pt 4), 1282-1293 (2009-04-01)
The periodontopathic bacterium Porphyromonas gingivalis forms pigmented colonies when incubated on blood agar plates as a result of accumulation of mu-oxo haem dimer on the cell surface. Gingipain-adhesin complexes are responsible for production of mu-oxo haem dimer from haemoglobin. Non-pigmented
Suikang Wang et al.
The Plant cell, 31(2), 486-501 (2019-01-25)
PROTEIN PHOSPHATASE4 (PP4) is a highly conserved Ser/Thr protein phosphatase found in yeast, plants, and animals. The composition and functions of PP4 in plants are poorly understood. Here, we uncovered the complexity of PP4 composition and function in Arabidopsis (Arabidopsis
Natalia Y Martynova et al.
Gene, 638, 52-59 (2017-09-18)
The homeodomain-containing transcription factor Anf (also known as Rpx/Hesx1 in mammals) plays an important role during the forebrain development in vertebrates. Here we demonstrate the ability of the Xenopus laevis Anf, Xanf1/Hesx1, to directly bind SRY-related HMG-box-containing transcription factor SoxD/Sox15
Haibo Du et al.
Neural plasticity, 2018, 2062346-2062346 (2018-05-26)
PDZD7 is an important deafness gene, whose mutations are associated with syndromic and nonsyndromic hearing loss. PDZD7 contains multiple PDZ domains that are essential for organizing various proteins into protein complex. Several PDZD7-binding proteins have been identified, including usherin, ADGRV1
Espen Aberg et al.
The Journal of biological chemistry, 284(29), 19392-19401 (2009-05-29)
ERK3 and ERK4 are atypical MAPKs in which the canonical TXY motif within the activation loop of the classical MAPKs is replaced by SEG. Both ERK3 and ERK4 bind, translocate, and activate the MAPK-activated protein kinase (MK) 5. The classical
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