一般描述
REDTaq®基因组DNA聚合酶是Taq DNA聚合酶与惰性红色染料的独特混合物。这种特殊的配方旨在增强更复杂或基因组模板的扩增。REDTaq基因组DNA聚合酶高度灵敏，可提高良率并延长产品长度。它具有REDTaq DNA聚合酶的所有优点，如易于观察加酶和完全反应混合，以及直接加载到琼脂糖凝胶中。这种惰性红色染料不会影响自动或手动测序、限制消化或其他下游应用。这种染料可以用任何标准的提纯方法轻易去除。
应用
REDTaq® Genomic DNA Polymerase has been used as a component of the polymerase chain reaction (PCR) mix for PCR amplification. It has also been used as a component of the preamplification mix for PCR in amplified fragment length polymorphism (AFLP) analysis of Fusarium oxysporum sp. isolates.
特点和优势
- 增强基因组和复杂 DNA 模板扩增
- 在实现与 Taq DNA 聚合酶相同性能的同时，具有适用于高通量应用的更方便形式
- 快速识别并确认适当混合
- 无需上样缓冲液或跟踪染料。样品可直接从反应中提取并上样至琼脂糖凝胶上
- 样品可以像嵌套PCR一样进行重复扩增
- 红色染料的迁移速度比溴酚蓝快
- 由于适当的混合，反应一致性更佳
组分
Includes 10× PCR reaction buffer without MgCl2 and a separate tube of 25 mM MgCl2
单位定义
在74°C下，一个单位可在30分钟内将10 nM总dNTP掺入酸可沉淀的DNA中。
法律信息
本产品的使用涵盖在以下一项或多项美国专利以及美国以外的相应专利要求中：美国 8,404,464 和美国7,972,828。本产品的采购包括上述专利要求下的有限、不可转让的诉讼豁免。
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
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实验方案
Reactions using REDTaq® DNA polymerase are formulated as any PCR mixtures. There are no additional reaction preparation steps or protocol changes required.
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