D6063
REDTaq® SuperPak™ DNA Polymerase
Taq for routine PCR with inert dye; with 10X buffer & dNTP mix
别名:
DNA polymerase with dNTP, DNA polymerase with loading dye, Taq DNA polymerase
产品名称
REDTaq® SuperPak™ DNA Polymerase, Taq for routine PCR with inert dye; with 10X buffer & dNTP mix
form
liquid
usage
sufficient for 250 reactions
feature
dNTPs included
hotstart: no
concentration
1 unit/μL
technique(s)
PCR: suitable
color
red
input
purified DNA
suitability
suitable for PCR
shipped in
wet ice
storage temp.
−20°C
Quality Level
Application
REDTaq® SuperPak™ DNA Polymerase has been used in:
- reverse transcriptase-polymerase chain reaction (RT-PCR)
- nested PCR
- routine PCR amplification
Features and Benefits
- Contains inert dye that enables visual confirmation of enzyme addition and direct loading onto agarose gel without the need for an additional loading dye
- Suitable for nested PCR
- Contains ultrapure 10mM dNTP mix in a separate vial
General description
REDTaq® SuperPak™ DNA Polymerase is Sigma′s Taq DNA Polymerase mixed with an inert red dye. REDTaq® SuperPak™ DNA Polymerase is a convenient package, that includes all the necessary components for a PCR reaction except primers, DNA template and water. It consists of Sigma′s high quality REDTaq DNA polymerase, 10 mM ultrapure deoxynucleotide mix and 10´ PCR reaction buffer. The dye provides quick recognition of reactions to which enzyme has been added as well as visual confirmation of complete mixing.
Legal Information
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
SuperPak is a trademark of Sigma-Aldrich Co. LLC
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
A Jóźwik et al.
Veterinary research communications, 29(4), 347-359 (2005-03-09)
Two pairs of primers were prepared, both localized within the sequences of the nucleoprotein gene (NP) of canine distemper virus (CDV). A number of experiments were done to optimize the conditions of RT-PCR and nested PCR methods. The nucleic acids
M Yeste-Velasco et al.
Neuroscience, 159(3), 1135-1147 (2009-04-10)
Increasing evidence implicates the c-Jun NH(2)-terminal kinase (JNK) pathway in the regulation of apoptosis in neurodegenerative diseases. In this study, we examined the neuroprotective effect of SP600125, a selective JNK inhibitor, in cerebellar granule cells (CGNs) deprived of serum and
J L Gonzales et al.
Veterinary parasitology, 146(1-2), 9-16 (2007-03-22)
Trypanosomosis caused by Trypanosoma vivax has been a constraint for cattle production in the Bolivian lowlands, since it was introduced in 1996. Flooded areas like the Bolivian Pantanal have a suitable environment for the presence and transmission of Salivarian trypanosomes
M Yeste-Velasco et al.
Neuropharmacology, 53(2), 295-307 (2007-07-07)
Recent studies have demonstrated that neuronal reentry in the cell cycle and specifically the expression of the transcription factor E2F-1, constitutes a pathway that may be involved in neuronal apoptosis after serum and potassium withdrawal. Other enzymes such as glycogen
D Alvira et al.
Neuroscience, 147(3), 746-756 (2007-06-23)
The mechanism involved in neuronal apoptosis is largely unknown. Studies performed on neuronal cell cultures provide information about the pathways which orchestrate the process of neuronal loss and potential drugs for the treatment of neurological disorders. In the present study
实验方案
Reactions using REDTaq® DNA polymerase are formulated as any PCR mixtures. There are no additional reaction preparation steps or protocol changes required.
Reviews the applications and benefits for RedTaq, including standard RedTaq, Hot Start RedTaq and RedTaq for genomic DNA PCR.
介绍RedTaq的应用和优势，包括用于基因组DNA PCR的标准RedTaq、热启动RedTaq和RedTaq。
相关内容
Instructions
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