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安全信息

CS1090

Sigma-Aldrich

HMG-CoA 还原酶检测试剂盒

sufficient for 30 assays (1 mL), sufficient for 100 assays (200 μL)

别名:

HMGR 检测试剂盒

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About This Item

UNSPSC代码:
12161503
NACRES:
NA.28

用途

sufficient for 100 assays (200 μL)
 sufficient for 30 assays (1 mL)

质量水平

200

运输

dry ice

储存温度

−70°C

基因信息

human ... HMGCR(3156)

相关类别

一般描述

HMG-CoA 还原酶测定试剂盒是胆固醇和其它相关代谢途径基础研究的重要工具。

应用

HMGR活性试剂盒可用于测定HMG-CoA还原酶活性。该试剂盒的主要功能是筛选HMG-CoA还原酶的纯化后催化亚基的不同抑制因子和激活因子，这在临床治疗中具有重要意义。该检测方法针对340nm处的吸光度下降进行光谱测量，而后者恰恰反映了存在HMG-CoA底物时HMGR催化亚基对NADPH的氧化水平。

生化/生理作用

HMGR（3-羟基-3-甲基戊二酰-CoA还原酶）是位于内质网上的一种跨膜糖蛋白。 这种酶可以催化HMG-CoA的四电子还原反应，生成辅酶A（CoA）和甲羟戊酸。这是固醇生物合成中的限速步骤。 为了维持衍生的甲基戊酸酯的浓度，可以通过合成、降解、磷酸化等方式控制HMGR的活性。除了HMGR的生理调控之外，人的HMGR酶可成功通过药物来靶定调控，可用于高血清胆固醇的临床治疗。 控制血清胆固醇体水平具有非常重要的治疗作用，这是因为血胆脂醇过多往往会引起动脉硬化病症加剧，进而引起心血管疾病，这可能会引起冠心病和中风。目前已有证据表明，胆固醇体内平衡受到干扰会引起慢性炎症恶化。

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • N6505β-Nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate, ≥95% (HPLC) 25 mg化学品安全说明书

储存分类代码

11 - Combustible Solids

法规信息

常规特殊物品
含少量动物源组分生物产品

分析证书(COA)

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. For Product CS1090, HMG-CoA Reductase Assay Kit, what is the concentration of kit component Product I5909, Pravastatin?

    The concentration of Pravastatin, Product I5909 in Product CS1090, HMG-CoA Reductase Assay Kit,  is 100 uM solution in assay buffer.

  6. What is the lowest concentration that you can use Pravastatin and observe inhibition in Product CS1090, HMG-CoA Reductase Assay kit?

    For Product CS1090, HMG-CoA Reductase Assay kit we do not observe any inhibition when using Pravastatin at 25 or 50 nM.  We observe inhibition of HMG-CoA Reductase with 250 nM pravastatin.

  7. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Rosana Aparecida Manólio Soares et al.
International journal of molecular sciences, 16(2), 4150-4160 (2015-02-19)
The objective of this study was to identify the major peptides generated by the in vitro hydrolysis of Amaranthus cruentus protein and to verify the effect of these peptides on the activity of 3-hydroxy-3-methyl-glutaryl-CoA reductase (HMG-CoA reductase), a key enzyme
Mohammad Azizur Rahman et al.
BioMed research international, 2014, 828149-828149 (2014-06-25)
Oxidation of low-density lipoprotein (LDL) has been strongly suggested as the key factor in the pathogenesis of atherosclerosis. Mushrooms have been implicated in having preventive effects against chronic diseases due especially to their antioxidant properties. In this study, in vitro
R Kleemann et al.
Current drug targets. Cardiovascular & haematological disorders, 5(6), 441-453 (2006-03-01)
Besides classical risk factors such as hypercholesterolemia and hypertension, chronic subacute inflammation has recently been recognized as an important force driving the development of atherosclerosis, the most common underlying cause of myocardial infarction and stroke. There is compelling evidence that
G A Holdgate et al.
Biochemical Society transactions, 31(Pt 3), 528-531 (2003-05-30)
The statins are inhibitors of 3-hydroxy-3-methylglutaryl CoA (HMG-CoA) reductase (HMG-CoAR), and are utilized to decrease levels of atherogenic lipoproteins in patients with, or who are at high risk of, cardiovascular disease. This study describes the inhibition of a recombinant, catalytic
A J Koning et al.
Molecular biology of the cell, 7(5), 769-789 (1996-05-01)
In all eucaryotic cell types analyzed, proliferations of the endoplasmic reticulum (ER) can be induced by increasing the levels of certain integral ER proteins. One of the best characterized of these proteins is HMG-CoA reductase, which catalyzes the rate-limiting step
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商品

HMG-CoA Reductase (HMGR) Assay Kit

3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) is a transmembrane glycoprotein, located on the endoplasmic reticulum.

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