一般描述
本品是由 对硝基苯基磷酸酯（pNPP）组成的缓冲碱性磷酸酶底物。这个先进的基质系统含有不溶性稳定剂，可提高稳定性和反应性，并降低基线吸收。它产生一种可溶性终产物，因此不推荐用于蛋白质印迹或组织化学应用。
应用
产生一种可溶性黄色反应产物，可在405nm处读取。对于终点测定，可以使用3N NaOH终止反应。推荐用于ELISA（微孔）分析；不建议用于膜应用。
注意
可在2-8°C下稳定保存至少一年。
外形
即用型
制备说明
含有对硝基苯基磷酸酯，它是碱性磷酸酶的底物。
警示用语：
Danger
危险声明
危险分类
Eye Dam. 1 - Skin Irrit. 2 - STOT RE 2
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Laura C Jacques et al.
Nature communications, 11(1), 1892-1892 (2020-04-22)
Streptococcus pneumoniae serotype 1 is the predominant cause of invasive pneumococcal disease in sub-Saharan Africa, but the mechanism behind its increased invasiveness is not well understood. Here, we use mouse models of lung infection to identify virulence factors associated with
Cristina A Teixeira et al.
Clinical science (London, England : 1979), 130(18), 1665-1675 (2016-07-08)
Transthyretin (TTR)-related amyloidoses are diseases characterized by extracellular deposition of amyloid fibrils and aggregates in tissues composed of insoluble misfolded TTR that becomes toxic. Previous studies have demonstrated the ability of small compounds in preventing and reversing TTR V30M deposition
Baoxin Wang et al.
Experimental and therapeutic medicine, 13(1), 178-186 (2017-01-27)
Damage to the recurrent laryngeal nerve often causes hoarseness, dyspnea, dysphagia, and sometimes asphyxia due to vocal cord paralysis which result in a reduction of quality of life. Brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) play
Hannah M Rowe et al.
Infection and immunity, 87(10) (2019-07-17)
Acute otitis media is one of the most common childhood infections worldwide. Currently licensed vaccines against the common otopathogen Streptococcus pneumoniae target the bacterial capsular polysaccharide and confer no protection against nonencapsulated strains or capsular types outside vaccine coverage. Mucosal
Xin-Yu Guo et al.
The Journal of biological chemistry, 295(48), 16393-16410 (2020-09-25)
The protein folding and lipid moiety status of glycosylphosphatidylinositol-anchored proteins (GPI-APs) are monitored in the endoplasmic reticulum (ER), with calnexin playing dual roles in the maturation of GPI-APs. In the present study, we investigated the functions of calnexin in the
商品
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
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