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Merck
CN

50933

盐酸胍 盐酸盐

BioUltra, Molecular Biology, ≥99.5% (AT)

别名:

亚氨基脲 盐酸盐, 亚氨脲 盐酸盐, 盐酸胍

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关于此项目

线性分子式:
NH2C(=NH)NH2 · HCl
化学文摘社编号:
50-01-1
分子量:
95.53
UNSPSC Code:
12352107
NACRES:
NA.28
PubChem Substance ID:
57651284
EC Number:
200-002-3
Beilstein/REAXYS Number:
3591990
MDL number:
MFCD00013026

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产品名称

盐酸胍 盐酸盐, BioUltra, Molecular Biology, ≥99.5% (AT)

InChI key

PJJJBBJSCAKJQF-UHFFFAOYSA-N

InChI

1S/CH5N3.ClH/c2-1(3)4;/h(H5,2,3,4);1H

SMILES string

Cl[H].NC(N)=N

biological source

synthetic

grade

Molecular Biology

product line

BioUltra

assay

≥99.5% (AT)

form

powder or crystals

technique(s)

RNA extraction: suitable

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected

ign. residue

≤0.05% (as SO4)

loss

≤0.05% loss on drying, 20 °C (HV)

color

colorless to white,

pH

4.5-6.0 (25 °C, 6 M in H2O)

mp

180-185 °C (lit.)

solubility

H2O: 6 M at 20 °C, clear, colorless

density

1.3 g/cm3 (lit.)

anion traces

sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤150 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

6 M in H2O

UV absorption

λ: 260 nm Amax: 0.03
λ: 280 nm Amax: 0.02

storage temp.

room temp

Quality Level

200

Gene Information

human ... KCNA1(3736), KCNA10(3744), KCNA2(3737), KCNA3(3738), KCNA4(3739), KCNA5(3741), KCNA6(3742), KCNA7(3743), KCNB1(3745), KCNB2(9312), KCNC1(3746), KCNC2(3747), KCNC3(3748), KCNC4(3749), KCND1(3750), KCND2(3751), KCND3(3752), KCNF1(3754), KCNG1(3755), KCNG2(26251), KCNG3(170850), KCNG4(93107), KCNH1(3756), KCNH2(3757), KCNH3(23416), KCNH4(23415), KCNH5(27133), KCNH6(81033), KCNH7(90134), KCNH8(131096), KCNQ1(3784), KCNQ2(3785), KCNQ3(3786), KCNQ4(9132), KCNQ5(56479), KCNS1(3787), KCNS2(3788), KCNS3(3790), KCNV1(27012), KCNV2(169522)

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相关类别

Application

强效离液剂，用于蛋白质的变性以及后续复性。该强效变性剂可溶解不溶性或变性的蛋白质，如包涵体。可用作使蛋白质或酶复性为活性形式的第一步骤。可能还需要脲和二硫苏糖醇 (DTT)。
盐酸胍(GdnHCl)已用作Schlemm管(SC)细胞孵育磷酸盐缓冲液的组分。还用作消化缓冲液组分，消化后的人HeLa229细胞可凝胶化并膨胀（进行膨胀显微镜观察）。盐酸胍还用于分离RNA。

Biochem/physiol Actions

盐酸胍(GdnHCl)可作为酶失活剂，导致酶的构象改变和活性丧失。它参与杂色鲍鱼体内的碱性磷酸酶(ALPase)失活。盐酸胍可在体内阻断热休克蛋白104（Hsp104）腺苷三磷酸酶(ATPase)的活性。还可令木瓜蛋白酶和氨基酰化酶等酶失活。较高浓度的盐酸胍可导致单纯疱疹病毒1型(HSV-1)等病毒灭活。

General description

盐酸胍(GdnHCl)是一种广泛使用的蛋白和酶变性剂。分子量小，且天然具有吸湿性。

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

存储类别

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000487184
0000487184
BCCN6783
0000433845
0000433845

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Isolation of RNA using guanidinium salts.
R J MacDonald et al.
Methods in enzymology, 152, 219-227 (1987-01-01)
Tobias C Kunz et al.
Frontiers in cellular and infection microbiology, 9, 276-276 (2019-08-27)
Expansion microscopy (ExM) is a novel tool to improve the resolution of fluorescence-based microscopy that has not yet been used to visualize intracellular pathogens. Here we show the expansion of the intracellular pathogen Chlamydia trachomatis, enabling to differentiate its two
Christa Meingast et al.
Biotechnology progress, 36(2), e2931-e2931 (2019-10-18)
Arginine synergistically inactivates enveloped viruses at a pH or temperature that does little harm to proteins, making it a desired process for therapeutic protein manufacturing. However, the mechanisms and optimal conditions for inactivation are not fully understood, and therefore, arginine
Current protocols in protein science, 84, A-A (2016-04-03)
The reagents and methods for purification and use of the most commonly used denaturants, guanidine hydrochloride (guanidine-HCl) and urea, are described. Other protein denaturants and reagents used to fold proteins are briefly mentioned. Sulfhydryl reagents (reducing agents) and "oxido-shuffling" (or
Jin Hua Liao et al.
Applied biochemistry and biotechnology, 158(2), 323-333 (2008-10-22)
Abalone, a kind of low poikilothermic invertebrate, is easily exposed to ocean environment stress. Since it is one of the important mariculture animals, the attention paid to the abalone study becomes increasing. Alkaline phosphatase (ALPase, EC 3.1.3.1) is a kind
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