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908401

Sigma-Aldrich

1-Methyl-7-nitroisatoic anhydride

别名:

1-Methyl-7-nitro-2H-3,1-benzoxazine-2,4(1H)-dione, 1-methyl-7-nitro-2H-3,1-Benzoxazine-2,4(1H)-dione, 1M7, RNA SHAPE probe, Reagent for RNA SHAPE-MaP

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About This Item

经验公式（希尔记法）:
C9H6N2O5
CAS号:
73043-80-8
分子量:
222.15
MDL编号:
MFCD18432729
UNSPSC代码:
12352119
NACRES:
NA.22

表单

powder

mp

204.5 °C

储存温度

2-8°C

SMILES字符串

[N+](=O)([O-])c1cc2[n]([c]([o][c](c2cc1)=O)=O)C

InChI

1S/C9H6N2O5/c1-10-7-4-5(11(14)15)2-3-6(7)8(12)16-9(10)13/h2-4H,1H3

InChI key

MULNCJWAVSDEKJ-UHFFFAOYSA-N

相关类别

应用

1-Methyl-7-nitroisatoic anhydride (1M7) is used as an in vivo SHAPE-MaP reagent for live cell RNA structure analysis at single nucleotide resolution. SHAPE -- or selective 2′-hydroxyl acylation analyzed by primer extension -- uses small, electrophilic chemical probes such as 1M7 to react with the 2′-hydroxyl group and provides insight to RNA structure. When combined with mutational profiling (MaP), quantitative nucleotide measurements are possible for entire transciptomes. Together, these methods deepen the understanding of RNA interactions and regions that may be exploited for design of RNA-targeting small-molecule drugs.

其他说明

Pervasive Regulatory Functions of mRNA Structure Revealed by High-Resolution SHAPE Probing

SnapShot: RNA Structure Probing Technologies

Detection of RNA-Protein Interactions in Living Cells with SHAPE

Standardization of RNA Chemical Mapping Experiments

In-cell RNA structure probing with SHAPE-MaP

A Fast-Acting Reagent for Accurate Analysis of RNA Secondary and Tertiary Structure by SHAPE Chemistry

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

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分析证书(COA)

Lot/Batch Number
MKCV6165
MKCP9847
MKCS2769
MKCN7060
MKCN1688

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Katherine E Deigan et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(1), 97-102 (2008-12-26)
Almost all RNAs can fold to form extensive base-paired secondary structures. Many of these structures then modulate numerous fundamental elements of gene expression. Deducing these structure-function relationships requires that it be possible to predict RNA secondary structures accurately. However, RNA
Matthew J Smola et al.
Biochemistry, 54(46), 6867-6875 (2015-11-07)
SHAPE-MaP is unique among RNA structure probing strategies in that it both measures flexibility at single-nucleotide resolution and quantifies the uncertainties in these measurements. We report a straightforward analytical framework that incorporates these uncertainties to allow detection of RNA structural
Jacob K Grohman et al.
Journal of the American Chemical Society, 133(50), 20326-20334 (2011-12-01)
Higher-order structure influences critical functions in nearly all noncoding and coding RNAs. Most single-nucleotide resolution RNA structure determination technologies cannot be used to analyze RNA from scarce biological samples, like viral genomes. To make quantitative RNA structure analysis applicable to
A fast-acting reagent for accurate analysis of RNA secondary and tertiary structure by SHAPE chemistry.
Stefanie A Mortimer et al.
Journal of the American Chemical Society, 129(14), 4144-4145 (2007-03-21)
Anthony M Mustoe et al.
Cell, 173(1), 181-195 (2018-03-20)
mRNAs can fold into complex structures that regulate gene expression. Resolving such structures de novo has remained challenging and has limited our understanding of the prevalence and functions of mRNA structure. We use SHAPE-MaP experiments in living E. coli cells to
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