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Merck
CN

C2230

Sigma-Aldrich

天然腔肠素

solid

别名:

3,2-二氢-2-(对羟基苄基)-6-(对羟基苯基)-8-苄基咪唑并 \ [1,2-a] 吡嗪-3-酮, CLZN

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About This Item

经验公式(希尔记法):
C26H21N3O3
CAS号:
分子量:
423.46
MDL编号:
UNSPSC代码:
12352200
PubChem化学物质编号:
NACRES:
NA.32

检测方案

≥95% (TLC)

形式

solid

溶解性

methanol and ethanol: soluble

储存温度

−20°C

SMILES字符串

Oc1ccc(CC2=NC3=C(Cc4ccccc4)NC(=CN3C2=O)c5ccc(O)cc5)cc1

InChI

1S/C26H21N3O3/c30-20-10-6-18(7-11-20)15-23-26(32)29-16-24(19-8-12-21(31)13-9-19)27-22(25(29)28-23)14-17-4-2-1-3-5-17/h1-13,16,27,30-31H,14-15H2

InChI key

YHIPILPTUVMWQT-UHFFFAOYSA-N

一般描述

腔肠素是萤光素分子,可被多种萤光素酶利用,如 海肾属刺虾属绿叶水母中存在的荧光素酶。

应用

天然腔肠素用于:
  • 水母发光蛋白测定
  • 在 COS-7 细胞中使用水母发光蛋白测量离子霉素或 PMA 诱导的细胞内钙离子浓度
  • 在生物发光成像中分析病毒依赖性荧光素酶活性的存在

生化/生理作用

当 Ca 2 + 与复合物结合时,水母发光蛋白复合物的发光基团被氧气氧化并在 465 nm 处发光;用于测量细胞内 Ca 2 + 浓度,灵敏度高,动态范围大。

注意

避免光照

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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