检测方案
≥95% (TLC)
形式
solid
溶解性
methanol and ethanol: soluble
储存温度
−20°C
SMILES字符串
Oc1ccc(CC2=NC3=C(Cc4ccccc4)NC(=CN3C2=O)c5ccc(O)cc5)cc1
InChI
1S/C26H21N3O3/c30-20-10-6-18(7-11-20)15-23-26(32)29-16-24(19-8-12-21(31)13-9-19)27-22(25(29)28-23)14-17-4-2-1-3-5-17/h1-13,16,27,30-31H,14-15H2
InChI key
YHIPILPTUVMWQT-UHFFFAOYSA-N
一般描述
腔肠素是萤光素分子,可被多种萤光素酶利用,如 海肾属、刺虾属 和绿叶水母中存在的荧光素酶。
应用
天然腔肠素用于:
- 水母发光蛋白测定
- 在 COS-7 细胞中使用水母发光蛋白测量离子霉素或 PMA 诱导的细胞内钙离子浓度
- 在生物发光成像中分析病毒依赖性荧光素酶活性的存在
生化/生理作用
当 Ca 2 + 与复合物结合时,水母发光蛋白复合物的发光基团被氧气氧化并在 465 nm 处发光;用于测量细胞内 Ca 2 + 浓度,灵敏度高,动态范围大。
注意
避免光照
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
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Secreted Gaussia luciferase (Gluc) has been shown to be a useful tool for ex vivo monitoring of in vivo biological processes. The Gluc level in the blood was used to detect tumor growth, metastasis and response to therapy, gene transfer
Protein expression and purification, 88(1), 150-156 (2013-01-01)
The cold-induced expression system in Escherichia coli is useful and we have applied this system to prepare the coelenterazine-utilizing luciferases including Renilla luciferase (RLase), a red-shifted variant of Renilla luciferase (RLase-547), the catalytic domain of Oplophorus luciferase (19kOLase) and Gaussia
Proceedings of the National Academy of Sciences of the United States of America, 108(29), 12060-12065 (2011-07-07)
Identifying protein-protein interactions (PPIs) is essential for understanding various disease mechanisms and developing new therapeutic approaches. Current methods for assaying cellular intermolecular interactions are mainly used for cells in culture and have limited use for the noninvasive assessment of small
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