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D7008

Sigma-Aldrich

Dihydroethidium

≥95% purity, powder

Synonym(s):

2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine, 3,8-Diamino-5,6-dihydro-5-ethyl-6-phenylphenanthridine, Hydroethidine

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25 MG
¥1,039.70
100 MG
¥3,528.82
500 MG
¥10,742.25
1 G
¥18,500.99

¥1,039.70

Available to ship on2025年4月14日Details

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25 MG
¥1,039.70
100 MG
¥3,528.82
500 MG
¥10,742.25
1 G
¥18,500.99

About This Item

Empirical Formula (Hill Notation):
C21H21N3
CAS Number:
104821-25-2
Molecular Weight:
315.41
Beilstein:
5107482
MDL number:
MFCD00077335
UNSPSC Code:
12171500
PubChem Substance ID:
24894069
NACRES:
NA.47

¥1,039.70

Available to ship on2025年4月14日Details

Request a Bulk Order

Product Name

Dihydroethidium, ≥95%

Quality Level

100

Assay

≥95%

form

powder

technique(s)

titration: suitable

color

pink to very dark red

solubility

chloroform: 20 mg/mL

fluorescence

λex 370 nm; λem 420 nm (for cytoplasm of living cells)
λex 535 nm; λem 610 nm (for chromatin of living cells)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

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Uridine 5′-diphospho-N-acetylglucosamine sodium salt ≥98%

U4375

Uridine 5′-diphospho-N-acetylglucosamine sodium salt

Uridine 5′-diphospho-N-acetylgalactosamine disodium salt ≥97%

U5252

Uridine 5′-diphospho-N-acetylgalactosamine disodium salt

Uridine 5′-diphosphogalactose disodium salt ≥97.0%

U4500

Uridine 5′-diphosphogalactose disodium salt

Uridine 5′-diphosphoglucuronic acid ammonium salt 98-100%

U5625

Uridine 5′-diphosphoglucuronic acid ammonium salt

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

powder

form

powder

form

powder

form

powder

assay

≥98%

assay

≥97%

assay

≥97.0%

assay

98-100%

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Application

Dihydroethidium has been used as a superoxide probe in bovine pulmonary artery endothelial cell (BPAEC).[1] It has been used to check the in situ generation of ROS (reactive oxygen species).[2][3]
Redox indicator. Blue fluorescence until oxidized to ethidium.

Packaging

Packaged under Argon.

Linkage

B-ring reduced analog of ethidium

related product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves
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    Lot/Batch Number
    MKCV6154
    MKCS1183
    MKCQ9818
    MKCN1192
    MKCL7544

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    Cira Rubies et al.
    Scientific reports, 9(1), 11443-11443 (2019-08-09)
    Obstructive sleep apnea syndrome (OSA) promotes aortic dilatation, increased stiffness and accelerated atherosclerosis, but the mechanisms of vascular remodelling are not known. We aimed to assess vascular remodelling, its mechanisms, and the effect of mesenchymal stem cells (MSC) infusions in
    Quercetin reduces oxidative stress and inhibits activation of c-Jun N-terminal kinase/activator protein-1 signaling in an experimental mouse model of abdominal aortic aneurysm.
    Wang L, et al.
    Molecular Medicine Reports, 9, 435-442 (2014)
    20-HETE increases superoxide production and activates NAPDH oxidase in pulmonary artery endothelial cells.
    Medhora M, et al.
    American Journal of Physiology. Lung Cellular and Molecular Physiology, 294, L902-L911 (2008)
    Rossarin Suwanarusk et al.
    The Journal of antimicrobial chemotherapy, 70(1), 124-129 (2014-08-26)
    Methylene blue, once discarded due to its unsettling yet mild side effects, has now found a renewed place in the pharmacopoeia of modern medicine. The continued spread of drug-resistant Plasmodium vivax and Plasmodium falciparum has also led to a recent
    Francisco R M Laurindo et al.
    Methods in enzymology, 441, 237-260 (2008-06-17)
    Assessment of low-level superoxide in nonphagocytic cells is crucial for assessing redox-dependent signaling pathways and the role of enzymes such as the NADPH oxidase complex. However, most superoxide probes present inherent limitations. Particularly, assessment of dihydroethidium (DHE) fluorescence is limited
    View All Related Papers

    Articles

    Mitochondrial Stress and ROS

    Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.

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