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35845

Sigma-Aldrich

DCFHDA

BioReagent, suitable for fluorescence, ≥95% (HPLC)

Synonym(s):

2′,7′-Dichlorofluorescein diacetate, Diacetyldichlorofluorescein

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About This Item

Empirical Formula (Hill Notation):
C24H14Cl2O7
CAS Number:
2044-85-1
Molecular Weight:
485.27
Beilstein:
68488
MDL number:
MFCD00037501
UNSPSC Code:
12352108
PubChem Substance ID:
57649438
NACRES:
NA.32

product line

BioReagent

Quality Level

100

Assay

≥95% (HPLC)

mp

232-234 °C (lit.)

solubility

DMSO: soluble

fluorescence

λex 504 nm; λem 524 nm in 0.1 M Tris pH 8.0 (esterase)

suitability

suitable for fluorescence

storage temp.

2-8°C

SMILES string

CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl

InChI

1S/C24H14Cl2O7/c1-11(27)30-21-9-19-15(7-17(21)25)24(14-6-4-3-5-13(14)23(29)33-24)16-8-18(26)22(31-12(2)28)10-20(16)32-19/h3-10H,1-2H3

InChI key

VQVUBYASAICPFU-UHFFFAOYSA-N

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General description

2′,7′-Dichlorofluorescein diacetate, also known as DCFHDA or Dcfh-DA, is a sensitive cell-permeable redox probe. The lipophilic non-fluorescent Dcfh-DA readily crosses the cell membrane through passive diffusion followed by deacetylation. The deacylated product is an oxidant sensitive 2′,7′-dichlorofluorescein (DCHF). DCHF is oxidized later to form highly fluorescent DCF, which is measured.

Application

2′,7′-Dichlorofluorescein diacetate is predominantly used to detect reactive oxygen (ROS) intermediates and quantify the overall oxidative stress in cells.

Dcfh-DA is used in the quantitative assay to measure oxidative stress in nanoparticle-treated cells.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is required to get 2´,7´-Dichlorofluorescein diacetate, Product 35845, to fluoresce?

    2',7'-dichlorofluorescein diacetate fluoresces only after cleavage of the acetyl groups.

  4. In what solvents is 2´,7´-´Dichlorofluorescein diacetate, Product 35845, soluble?

    It is soluble in DMF, ethanol (2 mg/mL), DMSO (10 mg/mL).

  5. What are the optical properties of the hydrolysis product of 2´,7´-Dichlorofluorescein diacetate, Product 35845?

    Excitation maximum is at 504 nm; emission maximum is at 529 nm (in 0.1 M Tris pH 8.0 following treatment with an esterase).

  6. What are the recommended storage conditions for 2´\,7´-Dichlorofluorescein diacetate, Product 35845?

    Product no. 35845 should be stored in a refrigerator or coldroom at 2-8°C.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. Can Product 35845, 2´,7´-Dichlorofluorescein diacetate be used for ROS (Reactive Oxygen Species) detection?

    Yes, Product 35845, 2´,7´-Dichlorofluorescein diacetate can be used for ROS detection by FACS analysis. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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