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Key Documents

287810

Sigma-Aldrich

2′,7′-Dichlorofluorescin Diacetate

Cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and nitric oxide (•NO) and for the determination of the degree of overall oxidative stress.

Synonym(s):

2′,7′-Dichlorofluorescin Diacetate, H₂DCFDA, 2ʹ,7ʹ-Dichlorodihydrofluorescein Diacetate

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CN¥9,493.39

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About This Item

Empirical Formula (Hill Notation):
C24H16Cl2O7
CAS Number:
4091-99-0
Molecular Weight:
487.29
MDL number:
MFCD00128955
UNSPSC Code:
12352202
NACRES:
NA.25

CN¥9,493.39

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Quality Level

100

Assay

≥94% (HPLC)

form

crystalline solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

solubility

DMSO: 5 mg/mL

shipped in

ambient

storage temp.

−20°C

SMILES string

Clc1cc2c(cc1OC(=O)C)Oc3c(cc(c(c3)OC(=O)C)Cl)C2c4c(cccc4)C(=O)O

InChI

1S/C24H16Cl2O7/c1-11(27)31-21-9-19-15(7-17(21)25)23(13-5-3-4-6-14(13)24(29)30)16-8-18(26)22(32-12(2)28)10-20(16)33-19/h3-10,23H,1-2H3,(H,29,30)

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technique(s)

GC/MS: suitable

technique(s)

HPLC: suitable

technique(s)

-

technique(s)

UV/Vis spectroscopy: suitable

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

assay

≥98% (GC)

assay

≥98% (GC)

assay

-

assay

≥99% (GC)

solubility

0.0095 g/L

solubility

-

solubility

-

solubility

-

form

liquid

form

liquid

form

-

form

liquid

vapor pressure

256 mmHg ( 37.7 °C), ~132 mmHg ( 20 °C), 5.2 psi ( 37.7 °C)

vapor pressure

256 mmHg ( 37.7 °C), 5.2 psi ( 37.7 °C), ~132 mmHg ( 20 °C)

vapor pressure

256 mmHg ( 37.7 °C), 5.2 psi ( 37.7 °C), ~132 mmHg ( 20 °C)

vapor pressure

256 mmHg ( 37.7 °C), 5.2 psi ( 37.7 °C), ~132 mmHg ( 20 °C)

General description

Cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and the nitric oxide (NO) in neutrophils and macrophages and for the determination of the overall oxidative stress in toxicological phenomena.

Biochem/physiol Actions

Cell permeable: yes
Primary Target
Detection of reactive oxygen species (ROS) and nitric oxide (•NO)
Product does not compete with ATP.
Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Crow, J.P. 1997. Nitric Oxide 1, 145.
Ripple, M.O., et al. 1997. J. Natl. Cancer Inst. 89, 40.
Caro, A., and Puntarulo, S. 1996. Biochim. Biophys. Acta1291, 245.
Perez-Garcia, F., et al. 1996. Life Sci. 59, 2033.
Ramakrishnan, N., et al. 1996. Biochem. Pharmacol. 51, 1443.
Ferrer, A.S., et al. 1990. Anal. Biochem. 187, 129.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

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