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D6883

Sigma-Aldrich

2′,7′-Dichlorodihydrofluorescein diacetate

≥97% (HPLC), powder, cell-permeable non-fluorescent probe

Synonym(s):

2′,7′-Dichlorofluorescin diacetate, DCFH-DA, 2′,7′-Dichlorodihydrofluorescein diacetate

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About This Item

Empirical Formula (Hill Notation):
C24H16Cl2O7
CAS Number:
4091-99-0
Molecular Weight:
487.29
Beilstein:
4610273
MDL number:
MFCD00128955
UNSPSC Code:
12352200
PubChem Substance ID:
24894058
NACRES:
NA.77

Product Name

2′,7′-Dichlorodihydrofluorescein diacetate, ≥97%

Quality Level

200

Assay

≥97%

form

powder

storage temp.

−20°C

SMILES string

CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C(c2cc1Cl)c4ccccc4C(O)=O

InChI

1S/C24H16Cl2O7/c1-11(27)31-21-9-19-15(7-17(21)25)23(13-5-3-4-6-14(13)24(29)30)16-8-18(26)22(32-12(2)28)10-20(16)33-19/h3-10,23H,1-2H3,(H,29,30)

InChI key

PXEZTIWVRVSYOK-UHFFFAOYSA-N

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General description

2′,7′-Dichlorodihydrofluorescein diacetate (DCFH-DA) is an oxidation-sensitive fluorescent dye used to detect the intracellular reactive oxygen species (ROS) level. DCFH-DA is a cell-permeable non-fluorescent probe, that can be hydrolyzed by intracellular esterase to DCFH, which forms fluorescent DCF when oxidized by the intracellular ROS.
The excitation wavelength is 504 nm and emission wavelength is 529 nm in ethanol. The use of DMSO as the solvent has not been determined, however various publications suggest that it is suitable.

Application

2′,7′-Dichlorofluorescin diacetate is suitable for use in:
  • sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism
  • microplate assay for detecting oxidative products in phagocytic cells
  • quantitative multiwell myeloid differentiation assay

Applications include sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism; microplate assay for detecting oxidative products in phagocytic cells, and quantitative multiwell myeloid differentiation assay.

Storage and Stability

This product should be stored in powdered format at -20°C.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000400210
0000352514
0000341644
0000335437
0000352514

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How should Product D6883, 2´,7´-Dichlorofluorescin diacetate, be dissolved?

    2´,7´-Dichlorofluorescin diacetate is soluble in organic solvents such as DMSO, ethanol, and DMF at 25 mg/mL. We test the solubility at 10 mg/ml in methanol.

  4. What is the solution stability of Product D6883, 2´,7´-Dichlorofluorescin diacetate?

    This product can be stored at -20°C for up to six months in solvents such as DMSO, ethanol, or DMF (N,N-dimethylformamide).

  5. What is the excitation and emission wavelength for Product D6883, 2´,7´-Dichlorofluorescin diacetate?

    The excitation wavelength is 504 nm and emission wavelength is 529 nm (in ethanol).

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

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