Skip to Content

尊敬的客户：

目前国际形势复杂多变，关税政策尚不明朗，这可能对我们的产品价格产生一定影响。在此情况下，我们希望就订单事宜与您进行友好沟通。

基于当前的不确定性，如果您选择在此期间下单，我们将保留根据实际情况调整价格的权利。同时，我们也理解市场变化可能给您带来的困扰，因此如果在订单实际发货前因关税政策变动导致价格出现较大波动，默克将与您进行协商讨论并视情况对订单进行调整或取消。

Merck
CN
All Photos(2)

Key Documents

View All Documentation

Safety Information

P4600

Sigma-Aldrich

ReadyMix™ Taq PCR Reaction Mix

with MgCl2

Synonym(s):

PCR master mix, Taq PCR master mix

Sign Into View Organizational & Contract Pricing

Select a Size

100 REACTIONS
¥2,468.27

¥2,468.27

Please contact Customer Service for Availability
Request a Bulk Order
All Photos(2)

Select a Size

Change View
100 REACTIONS
¥2,468.27

About This Item

MDL number:
MFCD01635810
UNSPSC Code:
41106300
NACRES:
NA.55

¥2,468.27

Please contact Customer Service for Availability
Request a Bulk Order

Quality Level

200

form

liquid

usage

sufficient for 100 reactions

feature

dNTPs included
hotstart: no

concentration

1.5 units/reaction (50 μL reaction volume)

technique(s)

PCR: suitable

color

colorless

input

purified DNA

application(s)

agriculture

shipped in

wet ice

Looking for similar products? Visit Product Comparison Guide

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
R2523D7440P1107
ReadyMix ™ Taq PCR 反应混合物 with MgCl2

P4600

ReadyMix ™ Taq PCR 反应混合物

REDTaq® ReadyMix™ PCR反应混合物 Complete PCR reagent with standard Taq DNA Polymerase and inert dye

R2523

REDTaq® ReadyMix™ PCR反应混合物

JumpStart™ Taq ReadyMix™预混液（定量PCR用） For probe-based real-time PCR

D7440

JumpStart™ Taq ReadyMix™预混液（定量PCR用）

JumpStart™ REDTaq® ReadyMix™ 反应混合物 for High-throughput PCR of complex templates

P1107

JumpStart™ REDTaq® ReadyMix™ 反应混合物

technique(s)

PCR: suitable

technique(s)

PCR: suitable

technique(s)

qPCR: suitable

technique(s)

PCR: suitable

form

liquid

form

liquid

form

liquid

form

liquid

usage

sufficient for 100 reactions

usage

sufficient for 100 reactions, sufficient for 20 reactions

usage

sufficient for 100 reactions, sufficient for 20 reactions, sufficient for 400 reactions

usage

sufficient for 100 reactions, sufficient for 400 reactions

feature

dNTPs included

feature

dNTPs included, hotstart: no

feature

Multiplex PCR, dNTPs included, hotstart

feature

Difficult Templates/Specialty Enzymes PCR, dNTPs included, hotstart

concentration

1.5 units/reaction (50 μL reaction volume)

concentration

-

concentration

2.5 units/reaction (50 μL reaction volume)

concentration

0.75 units/reaction (50 μL reaction volume)

color

colorless

color

red

color

colorless

color

red

General description

ReadyMix™ Taq PCR Reaction Mix is a prepared solution containing everything needed for a PCR reaction except the specific primers and template. The mix includes Sigma′s high quality Taq DNA Polymerase, 99% pure deoxynucleotides and buffer in a 2× optimized reaction concentrate. For reaction set-up, add the ReadyMix (25 μL) to the primers, template and water (total volume 50 μL). Using ReadyMix Taq PCR Reaction Mix reduces pipetting steps and risk of contamination. This saves time and reduces errors while still providing the great performance of Sigma′s Taq DNA Polymerase.

Application

ReadyMix™ Taq PCR Reaction Mix has been used:
  • in the development and specificity test of the amplified fragment length polymorphism (AFLP) markers[1]
  • in the PCR reaction for terminal restriction fragment length polymorphism (T-RFLP) analysis[2]
  • in the molecular identification of yeasts[3]
  • for routine PCR amplifications

Features and Benefits

  • Amplifies targets up to 7 kb in length

  • ReadyMix Taq PCR Reaction Mix offers the same great performance as Taq DNA Polymerase in a more convenient formulation

  • ReadyMix format reduces setup time.

  • Reduced risk of contamination from multiple pipet steps.

Packaging

Default reaction volume is 50 μL

100RXN is packaged as 1 X 2.5 mL
Supplied with a vial of PCR grade water for dilution.

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

related product

Product No.
Description
Pricing

Pictograms

Exclamation markEnvironment
GHS07,GHS09

Signal Word

Warning

Hazard Statements

H315,H317,H319,H410

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品
含少量动物源组分生物产品

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    0000369588
    0000364129
    0000369588
    0000364129
    0000344668

    Don't see the Right Version?

    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

    Search for a COA

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    Assessment of fecal microflora changes in pigs supplemented with herbal residue and prebiotic
    Samanta A K, et al.
    PLoS ONE, 10(7), e0132961-e0132961 (2015)
    Establishment of an in vitro trans-splicing system in Trypanosoma brucei that requires endogenous spliced leader RNA
    Shaked H, Wachtel C, Tulinski P, Yahia NH et al.
    Nucleic Acids Research, 38, 114-114 (2010)
    "RNA walk" a novel approach to study RNA?RNA interactions between a small RNA and its target.
    Lustig Y, Wachtel C, Safro M, Liu L and Michaeli S
    Nucleic Acids Research, 38, 5-5 (2010)
    Ashis Kumar Samanta et al.
    PloS one, 10(7), e0132961-e0132961 (2015-07-16)
    Antibiotic usage in animals as a growth promoter is considered as public health issue due to its negative impact on consumer health and environment. The present study aimed to evaluate effectiveness of herbal residue (ginger, Zingiber officinale, dried rhizome powder)
    Talla Sridhar Goud et al.
    Animal biotechnology, 31(4), 283-294 (2019-03-21)
    Melanocortin 1 receptor (MC1R) plays a vital role in melanogenesis and determines coat color of mammals. Polymorphic variants in MC1R, causing coat color variation, were described in few mammals; however, such studies were not done in cattle. The objective of
    View All Related Papers

    Articles

    Introduction & Historical Timelines

    Explore PCR's history, from discovery to Nobel Prize. Discover real-time PCR (qPCR) and digital PCR developments.

    PCR Master Mix

    PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.

    Polymerase Chain Reaction (PCR) Basics

    Polymerase chain reaction (PCR) is a technique that results in exponential amplification of a target DNA sequence.

    Protocols

    Standard PCR Protocol

    Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service