RDRT
ReadyScript® cDNA Synthesis Mix
Complete reagent for first strand cDNA synthesis for RT-qPCR
Synonym(s):
cDNA Synthesis Mix
About This Item
Recommended Products
usage
sufficient for 100 reactions
sufficient for 25 reactions
sufficient for 500 reactions
feature
dNTPs included
hotstart: no
technique(s)
RT-PCR: suitable
color
colorless
input
purified RNA
shipped in
dry ice
storage temp.
−20°C
Related Categories
1 of 4
This Item | SCC161 | SCC158 | SCC160 |
|---|---|---|---|
| technique(s) cell based assay: suitable, cell culture | mammalian: suitable | technique(s) cell based assay: suitable, cell culture | mammalian: suitable | technique(s) cell based assay: suitable, cell culture | mammalian: suitable | technique(s) cell based assay: suitable, cell culture | mammalian: suitable |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
General description
Application
Features and Benefits
- Convenient 5X master mix formula provides for quick and easy reaction set up; just add RNA template
- Includes proprietary blend of randomers and oligodT primers and an RNAse H+ derivative of Moloney murine leukemia virus (MMLV) reverse transcriptase
- Allows for unbiased, accurate conversion of RNA to cDNA in just 40 minutes
- Can use up to 1 μg of RNA in a single 20 μL reaction; reactions can also be scaled up
- Highly stable; can be stored at -20°C for up to 1 year; 4°C for up to one month
- Specific ratio of randomers and oligo (dT) primers in the mastermix ensures optimal representation of all transcript sequences in the cDNA product
- High reproducibility: Premixed master mix eliminates variability inherent in other kits requiring mixing of components
- RNase H(+) Moloney murine leukemia virus (MMLV) reverse transcriptase ensures residual RNA does not interfere with PCR amplification
- Reverse transcribes both rare and abundant genes with a wide input range of template
- Convenient 5X master mix formula provides for quick and easy reaction set up; just add RNA template
Components
Other Notes
Legal Information
recommended
related product
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Articles
Challenges in gene expression analysis include mRNA stability, temporal transcription patterns, and mRNA-protein correlation, impacting accuracy.
Sigma’s Imprint RNA Immunoprecipitation Kit was used to copurify human argonaute 2 (Ago2)-associated RNAs from HeLa cells. MicroRNAs reverse transcribed and quantitating using Mysticq reagents.
Protocols
Method for reverse transcription of RNA into DNA. Uses a premixed reagent that contains reverse transcriptase, dNTPs, primers, RNase inhibitor and buffer. Fast generation of cDNA.
Reverse transcription analyzes mRNA gene expression, facing challenges like transcript half-life differences and temporal patterns.
Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions
Related Content
Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.
RT-qPCR detects specific targets with applications in gene expression and pathogen detection.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
