GE27-9261-01
First-strand cDNA Synthesis Kit
Cytiva 27-9261-01
Synonym(s):
cDNA Synthesis Kit
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¥1,321.90
Select a Size
About This Item
¥1,321.90
Recommended Products
manufacturer/tradename
Cytiva 27-9261-01
color
colorless
shipped in
dry ice
storage temp.
−20°C
1 of 4
This Item | RAB0904 | RAB0277 | RAB0278 |
|---|---|---|---|
| species reactivity rat | species reactivity rat | species reactivity rat | species reactivity rat |
| Gene Information rat ... Inhba(29200) | Gene Information rat ... Ins1(24505) | Gene Information rat ... Il1b(24494) | Gene Information rat ... Il1b(24494) |
| assay range inter-assay cv: <12% | assay range inter-assay cv: <12% | assay range inter-assay cv: <12% | assay range inter-assay cv: <12% |
| input sample type plasma | input sample type serum | input sample type cell culture supernatant(s) | input sample type cell lysate |
| detection method colorimetric | detection method colorimetric | detection method colorimetric | detection method colorimetric |
| technique(s) ELISA: suitable | technique(s) ELISA: suitable | technique(s) ELISA: suitable, capture ELISA: suitable | technique(s) ELISA: suitable, capture ELISA: suitable |
General description
First-Strand cDNA Synthesis Kit is designed to generate full-length first-strand cDNA from mRNA templates. Following first-strand cDNA synthesis, the sample can be used directly for in vitro amplification using PCR or for second-strand synthesis using the Gubler-Hoffman method. Primers are not included in the preassembled First-Strand Reaction Mixes, thus allowing use of a primer of choice. Two primers are included with the kit. pd(N)6 Primer (random hexamer) is used to prepare libraries for screening with antibodies, to increase the representation of 5′-ends of mRNAs having significant secondary structure, or to copy mRNAs lacking a poly(A) tail.
The NotI-(dT)18 Bifunctional Primer can be used to prime selectively on mRNA that has a poly(A) tail. It can also be used as a PCR primer following first-strand synthesis. The NotI restriction site, located at the 5′-end of the primer, is particularly useful for subsequent directional cloning of cDNA and PCR products.
First-Strand cDNA Synthesis Kit contains the following reagents, sufficient for up to 55 first-strand syntheses, each of which will produce enough material for up to 15 amplification reactions: Bulk First-Strand cDNA Reaction Mixes, mRNA Standard, DTT Solution, RNase-Free Water, pd(N)6 Primer, NotI-(dT)18 Bifunctional Primer, and instruction booklet.
Application
Features and Benefits
- For synthesis of single-stranded cDNA prior to amplification by PCR.
- Features preassembled bulk reaction mixes to save time and minimize potential for pipetting errors.
- Allows users to choose primers.
- Includes a choice of two primers: pd(N)6 random hexamers and NotI-(dT)18.
Storage and Stability
Analysis Note
Storage Class Code
12 - Non Combustible Liquids
Regulatory Information
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