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11855476001
Roche
Titan One Tube RT-PCR System
sufficient for 100 reactions, kit of 1 (4 components), suitable for RT-PCR, hotstart: no
Synonym(s):
RT-PCR
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10 X 1 L
CN¥1,981.71
CN¥1,981.71
List priceCN¥2,831.02Save 30%Please contact Customer Service for Availability
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10 X 1 L
CN¥1,981.71
About This Item
UNSPSC Code:
41106313
CN¥1,981.71
List priceCN¥2,831.02Save 30%Please contact Customer Service for Availability
Recommended Products
usage
sufficient for 100 reactions
Quality Level
feature
dNTPs included: no
hotstart: no
packaging
kit of 1 (4 components)
manufacturer/tradename
Roche
parameter
42-60 °C optimum reaction temp.
technique(s)
RT-PCR: suitable
input
purified RNA
storage temp.
−20°C
Related Categories
1 of 4
This Item | K3753 | T2397 | H1387 |
|---|---|---|---|
| Quality Level 500 | Quality Level 500 | Quality Level 400 | Quality Level 500 |
| form powder | form powder | form liquid | form powder |
| pH 6.1-6.7 | pH - | pH - | pH - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. - | storage temp. 2-8°C |
| solubility water: 9.5 g/L, clear | solubility - | solubility - | solubility - |
General description
The Titan™ One Tube RT-PCR System uses Reverse Transcriptase (RT) Avian Myeloblastosis Virus (AMV) for first strand cDNA synthesis and the Expand™ High Fidelity enzyme blend consisting of Taq DNA Polymerase and a polymerase with a proofreading activity for amplification of cDNA by polymerase chain reaction (PCR). The proofreading activity, leads to an increased PCR fidelity. The Titan One Tube RT-PCR System:
- Reduces the error rate in PCR due to the proofreading capability. A threefold higher fidelity is obtained in comparision to Taq DNA polymerase.
- Delivers increased sensitivity due to the high efficiency of all three enzymes.
- Is faster than conventional two-step RT-PCR.
Specificity
Specificity of the reaction strongly depends on primer design and DNA contamination.
To exclude artefacts from DNA targets appropriate positive and negative control reaction should be included.
Heat inactivation: 2 min at 94 °C
Reverse Transcriptase AMV
To exclude artefacts from DNA targets appropriate positive and negative control reaction should be included.
Heat inactivation: 2 min at 94 °C
Reverse Transcriptase AMV
Application
Titan™ One Tube RT-PCR System has been used:
Features and Benefits
Titan™ One Tube RT-PCR System:
- High Fidelity: Expand™ High Fidelity enzyme blend with proofreading activity reduces the error rate in polymerase chain reaction (PCR)
- offers a threefold higher fidelity in comparison to Taq DNA polymerase
- delivers increased sensitivity due to the high efficiency of all three enzymes
- faster than conventional two-step reverse transcriptase (RT)-PCR
- allows the amplification of full-length cDNA up to 6 kb by RT-PCR due to the use of the Expand™ PCR System
Packaging
1 kit containing 4 components
Other Notes
For life science research only. Not for use in diagnostic procedures.
Legal Information
Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under such patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.</ br>NOTICE TO PURCHASER: LIMITED LICENSE<br />Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche. All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany. Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.
Expand is a trademark of Roche
Titan is a trademark of Roche
Kit Components Only
Product No.
Description
- Reverse Transcriptase AMV and Expand High Fidelity Enzyme Mix in storage buffer
- RT-PCR Reaction Buffer 5x concentrated
- MgCl2 Stock Solution, 25 mM
- DTT Solution, 100 mM
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3 - Eye Irrit. 2
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Regulatory Information
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Yogesh R Suryawanshi et al.
Molecular therapy oncolytics, 23, 1-13 (2021-10-01)
A dual microRNA-detargeted oncolytic Mengovirus, vMC24NC, proved highly effective against a murine plasmacytoma in an immunocompetent syngeneic mouse model; however, there remains the concern of escape mutant development and the potential for toxicity in severely immunocompromised cancer patients when it
Autumn J Ruiz et al.
Journal of virology, 90(8), 4078-4092 (2016-02-13)
Mengovirus, a member of thePicornaviridaefamily, has a broad cell tropism and can cause encephalitis and myocarditis in multiple mammalian species. Attenuation has been achieved by shortening the polycytidine tract in the 5' noncoding region (NCR). A poly(C)-truncated strain of mengovirus
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