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HPLC Analysis of Drugs of Abuse on Ascentis® Express F5

HPLC Analysis of Drugs of Abuse on Ascentis® Express F5 application for HPLC

材料

分析色谱柱

产品编号
说明
价格

53969-U

Ascentis® Express HILIC, 2.7 μm HPLC色谱柱

2.7 μm particle size, L × I.D. 7.5 cm × 3 mm

标准

产品编号
说明
价格

C5901

可待因 一水合物

analytical standard

44179

吗啡 硫酸盐 五水合物

analytical standard

B-007

苯甲酰芽子碱标准液 溶液

100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

F-002

芬太尼标准液 溶液

100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

H-003

氢可酮标准液 溶液

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

M-013

3,4-亚甲基二氧基甲基苯丙胺标准液（MDMA） 溶液

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

M-022

(±)-甲基苯丙胺标准液 溶液

100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

M-035

度冷丁标准液 溶液

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

N-017

去甲哌替啶标准液 溶液

100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

P-011

(+)-丙氧芬 溶液

1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®

CONDITIONS

column

Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U)

mobile phase

[A] 5 mM ammonium formate; [B] 5 mM ammonium formate in 95:5 (v/v) acetonitrile:water

gradient

held at 20% B for 0.5 min; 20 to 100% B in 5.5 min; held at 100% B for 4 min

flow rate

0.3 mL/min

pressure

175 bar

column temp.

35 °C

detector

ESI(+), m/z 100-1000

injection

1 μL

sample

1 μg/mL in 50:50, water: methanol

说明

法律信息

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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