跳转至内容

Dear Customer:

The current international situation is complex and volatile, and uncertain tariff policies may potentially impact our product prices. Given these uncertainties, we value your understanding regarding order-related matters.

If you decide to place an order during this period, we reserve the right to adjust the price based on the evolving situation. We understand that market changes may cause inconvenience. We will negotiate with you if there’s a significant price fluctuation due to tariff policy changes before the order’s actual delivery, and in such cases we may adjust or cancel the order as necessary.

Merck
CN
  • Exosomes from human immunodeficiency virus type 1 (HIV-1)-infected cells license quiescent CD4+ T lymphocytes to replicate HIV-1 through a Nef- and ADAM17-dependent mechanism.

Exosomes from human immunodeficiency virus type 1 (HIV-1)-infected cells license quiescent CD4+ T lymphocytes to replicate HIV-1 through a Nef- and ADAM17-dependent mechanism.

Journal of virology (2014-07-25)
Claudia Arenaccio, Chiara Chiozzini, Sandra Columba-Cabezas, Francesco Manfredi, Elisabetta Affabris, Andreas Baur, Maurizio Federico
摘要

Resting CD4+ T lymphocytes resist human immunodeficiency virus (HIV) infection. Here, we provide evidence that exosomes from HIV-1-infected cells render resting human primary CD4+ T lymphocytes permissive to HIV-1 replication. These results were obtained with transwell cocultures of HIV-1-infected cells with quiescent CD4+ T lymphocytes in the presence of inhibitors of exosome release and were confirmed using exosomes purified from supernatants of HIV-1-infected primary CD4+ T lymphocytes. We found that the expression of HIV-1 Nef in exosome-producing cells is both necessary and sufficient for cell activation as well as HIV-1 replication in target CD4+ T lymphocytes. We also identified a Nef domain important for the effects we observed, i.e., the 62EEEE65 acidic cluster domain. In addition, we observed that ADAM17, i.e., a disintegrin and metalloprotease converting pro-tumor necrosis factor alpha (TNF-α) in its mature form, associates with exosomes from HIV-1-infected cells, and plays a key role in the HIV-1 replication in quiescent CD4+ T lymphocytes. Treatment with an inhibitor of ADAM17 abolished both activation and HIV-1 replication in resting CD4+ T lymphocytes. TNF-α is the downstream effector of ADAM17 since the treatment of resting lymphocytes with anti-TNF-α antibodies blocked the HIV-1 replication. The data presented here are consistent with a model where Nef induces intercellular communication through exosomes to activate bystander quiescent CD4+ T lymphocytes, thus stimulating viral spread. Overall, our findings support the idea that HIV evolved to usurp the exosome-based intercellular communication network to favor its spread in infected hosts.

材料
货号
品牌
产品描述

Sigma-Aldrich
荧光素异硫氰酸酯异构体I, suitable for protein labeling, ≥90% (HPLC), powder
登录查看公司和协议定价
货号包装规格是否有货价格数量
1 kit
国内现货,预计发货时间 2025年4月15日
详情...
¥5,251.95
Sigma-Aldrich
荧光素 5(6)-异硫氰酸酯, BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)
登录查看公司和协议定价
货号包装规格是否有货价格数量
1 kit
国内现货,预计发货时间 2025年4月15日
详情...
¥5,251.95
Sigma-Aldrich
单克隆抗 β-肌动蛋白抗体 小鼠抗, clone AC-74, ascites fluid
登录查看公司和协议定价
货号包装规格是否有货价格数量
1 kit
国内现货,预计发货时间 2025年4月15日
详情...
¥5,251.95
Sigma-Aldrich
白细胞介素-2 人, IL-2, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
登录查看公司和协议定价
货号包装规格是否有货价格数量
1 kit
国内现货,预计发货时间 2025年4月15日
详情...
¥5,251.95
Sigma-Aldrich
荧光素 5(6)-异硫氰酸酯, ≥90% (HPLC)
登录查看公司和协议定价
货号包装规格是否有货价格数量
1 kit
国内现货,预计发货时间 2025年4月15日
详情...
¥5,251.95
Sigma-Aldrich
荧光素异硫氰酸酯异构体I, ≥97.5% (HPLC)
登录查看公司和协议定价
货号包装规格是否有货价格数量
1 kit
国内现货,预计发货时间 2025年4月15日
详情...
¥5,251.95
Sigma-Aldrich
白细胞介素-2 人, IL-2, recombinant, expressed in HEK 293 cells, suitable for cell culture, endotoxin tested
登录查看公司和协议定价
货号包装规格是否有货价格数量
1 kit
国内现货,预计发货时间 2025年4月15日
详情...
¥5,251.95
Sigma-Aldrich
白细胞介素-2 人, recombinant, expressed in Pichia pastoris, suitable for cell culture
登录查看公司和协议定价
货号包装规格是否有货价格数量
1 kit
国内现货,预计发货时间 2025年4月15日
详情...
¥5,251.95
Sigma-Aldrich
白细胞介素-2 人, recombinant, expressed in E. coli, ~10000 U/mL
登录查看公司和协议定价
货号包装规格是否有货价格数量
1 kit
国内现货,预计发货时间 2025年4月15日
详情...
¥5,251.95
Sigma-Aldrich
人白细胞介素-2 人, Animal-component free, recombinant, expressed in E. coli, suitable for cell culture
登录查看公司和协议定价
货号包装规格是否有货价格数量
1 kit
国内现货,预计发货时间 2025年4月15日
详情...
¥5,251.95
Sigma-Aldrich
MISSION® esiRNA, targeting human IL2
登录查看公司和协议定价
货号包装规格是否有货价格数量
1 kit
国内现货,预计发货时间 2025年4月15日
详情...
¥5,251.95