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产品名称
荧光素异硫氰酸酯异构体I, suitable for protein labeling, ≥90% (HPLC), powder
质量水平
方案
≥90% (HPLC)
表单
powder
技术
titration: suitable
颜色
orange to dark orange
mp
>360 °C (lit.)
溶解性
acetone: 1 mg/mL
荧光
λex 492 nm; λem 518 nm (green)
适用性
suitable for protein labeling
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此商品 | BCR546 | CRM4M7177 | CRM47177 |
|---|---|---|---|
| technique(s) HPLC: suitable, gas chromatography (GC): suitable | technique(s) - | technique(s) HPLC: suitable, gas chromatography (GC): suitable | technique(s) HPLC: suitable, gas chromatography (GC): suitable |
| application(s) environmental | application(s) environmental | application(s) environmental | application(s) environmental |
| format neat | format neat | format single component solution | format single component solution |
| grade analytical standard | grade certified reference material | grade certified reference material, TraceCERT® | grade certified reference material, TraceCERT® |
| storage temp. 2-30°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| packaging ampule of 100 mg | packaging - | packaging ampule of 5 × 1 mL | packaging ampule of 1 mL |
一般描述
警示用语：
Danger
危险声明
危险分类
Resp. Sens. 1 - Skin Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
历史批次信息供参考:
分析证书(COA)
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
After the FITC (e.g., Product No. F7250) is conjugated to the protein, what is the solution stability of the conjugated protein?
The stability of the conjugated protein will be essentially the stability of the unconjugated protein. Some conjugated proteins might be stable when stored at -20°C and other conjugated proteins will have better solution stability at 2-8°C If the protein concentration is too low, this also has a negative effect upon the stability of the conjugated protein.
Is Product No. F7250, Fluorescein isothiocyanate isomer I, pharmaceutical grade?
No. The product is considered suitable for research use only.
How stable is FITC Isomer 1 (e.g., Product No. F7250) once conjugated to an antibody?
FITC can photo bleach fairly rapidly. The prepared solution should be stored in a dark container and protected from light. When viewing the antibody staining under a microscope, it is advisable to use subdued fluorescent light when possible. If the fluorescent light stays at one position for too long, photo bleaching is noticeable.
Is the intensity of FITC (e.g., Product No. F7250) pH dependent?
Yes; many articles have reported the pH dependence of the fluorescence of fluorescein derivatives. See the following references: Archives Biochem. Biophys., 133, 263 (1969); J. Immunol., 95, 1165 (1966); Archives Biochem. Biophys., 73, 1 (1958); Standard test for labeling efficiency of FITC with BSA: NCCLS Approved Standard: ASM-1, 2nd ed., June, 1975; Characterization of FITC-dyes by IR and TLC: Analytical Biochem., 29, 526 (1969); Fluorescent labeling of proteins: Proc. Nat. Acad. Sci., 72, 459 (1975); Amer. J. Pathol., 34, 1081 (1958); Fluorescent labeling of a lectin: Meth. Enzymol., 81, 65 (1982).
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