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Merck
CN

46950

Sigma-Aldrich

荧光素 5(6)-异硫氰酸酯

BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)

别名:

FITC, 异硫氰酸荧光素

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About This Item

经验公式(希尔记法):
C21H11NO5S
CAS号:
分子量:
389.38
Beilstein:
1407295
EC 号:
MDL编号:
UNSPSC代码:
12171500
PubChem化学物质编号:
NACRES:
NA.32

质量水平

产品线

BioReagent

检测方案

≥90% (HPLC)

荧光

λex 492 nm; λem 518 nm in 0.1 M phosphate pH 8.0

适用性

suitable for fluorescence

储存温度

−20°C

SMILES字符串

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1

InChI

1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H

InChI key

MHMNJMPURVTYEJ-UHFFFAOYSA-N

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一般描述

荧光素5(6)-异硫氰酸酯,也称为FITC,是一种弱酸性的羟基黄酮类化合物,其整体尺寸较小,在苯环上具有羧酸和异硫氰酸酯基团。这种染料具有中等大小、非平面的芳香环系。 异硫氰酸酯基团与生物分子的亲核官能团(如氨基,羟基,巯基)反应,作为荧光标记物附加在生物分子上。 该染料在相同溶剂中的最大发射波长为519纳米。

应用

荧光素5(6)-异硫氰酸(FITC)标记的分子用于非显微免疫测定和生理示踪实验。FITC在流式细胞术实验中广泛用于蛋白质、细胞核和亚核颗粒的染色。 使用FITC标记的白细胞(WBC)可用于研究其对趋化因子的反应性并进行最终测定。
用于蛋白质的荧光标记(例如免疫荧光);凝胶色谱中的 H 蛋白质标记

包装

无底玻璃瓶。内容物在插入的熔锥内。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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