46950
荧光素 5(6)-异硫氰酸酯
BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)
别名:
FITC, 异硫氰酸荧光素
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关于此项目
经验公式（希尔记法）:
C21H11NO5S
化学文摘社编号:
分子量:
389.38
UNSPSC Code:
12171500
NACRES:
NA.32
PubChem Substance ID:
EC Number:
248-207-7
Beilstein/REAXYS Number:
1407295
MDL number:
Quality Level
InChI key
MHMNJMPURVTYEJ-UHFFFAOYSA-N
InChI
1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
SMILES string
Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
product line
BioReagent
assay
≥90% (HPLC)
form
powder
mol wt
389.38 g/mol
color
orange to dark orange, yellow to dark yellow
solubility
acetone: 0.1 g/10 mL, clear to very slightly hazy
fluorescence
λex 492 nm; λem 518 nm in 0.1 M phosphate pH 8.0
suitability
suitable for fluorescence
storage temp.
−20°C
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General description
荧光素5（6）-异硫氰酸酯，也称为FITC，是一种弱酸性的羟基黄酮类化合物，其整体尺寸较小，在苯环上具有羧酸和异硫氰酸酯基团。这种染料具有中等大小、非平面的芳香环系。 异硫氰酸酯基团与生物分子的亲核官能团（如氨基，羟基，巯基）反应，作为荧光标记物附加在生物分子上。 该染料在相同溶剂中的最大发射波长为519纳米。
Application
用于蛋白质的荧光标记（例如免疫荧光）;凝胶色谱中的 H 蛋白质标记
荧光素5(6)-异硫氰酸(FITC)标记的分子用于非显微免疫测定和生理示踪实验。FITC在流式细胞术实验中广泛用于蛋白质、细胞核和亚核颗粒的染色。 使用FITC标记的白细胞（WBC）可用于研究其对趋化因子的反应性并进行最终测定。
Packaging
无底玻璃瓶。内容物在插入的熔锥内。
存储类别
11 - Combustible Solids
wgk
WGK 3
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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