跳转至内容
Merck
CN
  • FOXM1 confers to epithelial-mesenchymal transition, stemness and chemoresistance in epithelial ovarian carcinoma cells.

FOXM1 confers to epithelial-mesenchymal transition, stemness and chemoresistance in epithelial ovarian carcinoma cells.

Oncotarget (2014-12-30)
Wen-Tai Chiu, Yu-Fang Huang, Huei-Yu Tsai, Chien-Chin Chen, Chang-Hwa Chang, Soon-Cen Huang, Keng-Fu Hsu, Cheng-Yang Chou
摘要

Chemoresistance to anti-cancer drugs substantially reduces survival in epithelial ovarian cancer. In this study, we showed that chemoresistance to cisplatin and paclitaxel induced the epithelial-mesenchymal transition (EMT) and a stem cell phenotype in ovarian cancer cells. Chemoresistance was associated with the downregulation of epithelial markers and the upregulation of mesenchymal markers, EMT-related transcription factors, and cancer stem cell markers, which enhanced invasion and sphere formation ability. Overexpression of FOXM1 increased cisplatin-resistance and sphere formation in cisplatin-sensitive and low FOXM1-expressing ovarian cancer cells. Conversely, depletion of FOXM1 via RNA interference reduced cisplatin resistance and sphere formation in cisplatin-resistant and high FOXM1-expressing cells. Overexpression of FOXM1 also increased the expression, nuclear accumulation, and activity of β-CATENIN in chemoresistant cells, whereas downregulation of FOXM1 suppressed these events. The combination of cisplatin and the FOXM1 inhibitor thiostrepton inhibited the expression of stem cell markers in chemoresistant cells and subcutaneous ovarian tumor growth in mouse xenografts. In an analysis of 106 ovarian cancer patients, high FOXM1 levels in tumors were associated with cancer progression and short progression-free intervals. Collectively, our findings highlight the importance of FOXM1 in chemoresistance and suggest that FOXM1 inhibitors may be useful for treatment of ovarian cancer.

材料
货号
品牌
产品描述

Sigma-Aldrich
N,N-二甲基乙酰胺, suitable for HPLC, ≥99.9%
Sigma-Aldrich
甲醛 溶液, for molecular biology, 36.5-38% in H2O
Sigma-Aldrich
噻唑蓝, 98%
Sigma-Aldrich
N,N-二甲基乙酰胺, puriss. p.a., ≥99.5% (GC)
Sigma-Aldrich
噻唑蓝, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)
SAFC
甲醛 溶液, contains 10-15% methanol as stabilizer, 37 wt. % in H2O
Sigma-Aldrich
N,N-二甲基乙酰胺, ReagentPlus®, 99%
Sigma-Aldrich
N,N-二甲基乙酰胺, ReagentPlus®, ≥99%
Sigma-Aldrich
苯均三酸, 95%
Sigma-Aldrich
甲醛 溶液, for molecular biology, BioReagent, ≥36.0% in H2O (T)
Sigma-Aldrich
L-赖氨酸 单盐酸盐, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 98.5-101.0%
Sigma-Aldrich
顺铂, crystalline
Supelco
甲醛 溶液, stabilized with methanol, ~37 wt. % in H2O, certified reference material
Sigma-Aldrich
甲醛 溶液, ACS reagent, 37 wt. % in H2O, contains 10-15% Methanol as stabilizer (to prevent polymerization)
Sigma-Aldrich
二氯化二胺(II), ≥99.9% trace metals basis
Sigma-Aldrich
N,N-二甲基乙酰胺, suitable for peptide synthesis, ≥99.8% (GC)
Sigma-Aldrich
N,N-二甲基乙酰胺-d9, 99 atom % D
Sigma-Aldrich
甲醛 溶液, meets analytical specification of USP, ≥34.5 wt. %
Sigma-Aldrich
N,N-二甲基乙酰胺, spectrophotometric grade, ≥99%
Sigma-Aldrich
N,N-二甲基乙酰胺, anhydrous, 99.8%
Sigma-Aldrich
甲醛 溶液, tested according to Ph. Eur.
Sigma-Aldrich
L-赖氨酸 单盐酸盐, reagent grade, ≥98% (HPLC)
Sigma-Aldrich
反式-二氨二氯合铂(II)
Sigma-Aldrich
甲醛-12C 溶液, 20% in H2O, 99.9 atom % 12C
SAFC
N,N-二甲基乙酰胺, Ph. Eur.
顺氯氨铂, European Pharmacopoeia (EP) Reference Standard
USP
反式-二氨二氯合铂(II), United States Pharmacopeia (USP) Reference Standard
Supelco
L-赖氨酸 单盐酸盐, Pharmaceutical Secondary Standard; Certified Reference Material
赖氨酸 盐酸盐, European Pharmacopoeia (EP) Reference Standard
Supelco
N,N-二甲基乙酰胺, analytical standard