HPLC Analysis of Pain Relievers on Ascentis^® Express C18

技术

HPLC: suitable

试验参数

column: Ascentis Express C18, 10 cm x 4.6 mm I.D., 5 μm particles (50536-U)
mobile phase: [A] water with 3% acetic acid; [B] methanol with 3% acetic acid; (79:21, A:B)
flow rate: 2.0 mL/min
pressure: 3290 psi (227 bar)
column temp.: 45 °C
detector: UV,275 nm
injection: 10 μL
sample: 100 - 360 μg/mLin mobile phase

适用性

application for HPLC

应用

clinical
pharmaceutical (small molecule)