LC/MS Analysis of L-Methionine Sulfoximine and Sulfone on Ascentis^® Express OH5

Name: LC/MS Analysis of L-Methionine Sulfoximine and Sulfone on Ascentis<SUP>®</SUP> Express OH5
Brand: SUPELCO

application for HPLC

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Sigma-Aldrich

M5379

L-蛋氨酸亚砜酰亚胺

G005790

LC/MS Analysis of L-Methionine Sulfoximine, Sulfone and Sulfoxide on Ascentis^® Express HILIC

SAFC

104309

L-蛋氨酸亚砜酰亚胺

Sigma-Aldrich

GSS-1015

GS系统100mM L-甲硫氨酸亚砜亚胺，工作范围25 µ M-1mM

SAFC

76078

L-蛋氨酸亚砜酰亚胺

Sigma-Aldrich

MAK314

唾液酸检测试剂盒

Supelco

91016

L-蛋氨酸亚砜酰亚胺

Sigma-Aldrich

SIALICQ

唾液酸定量试剂盒

Sigma-Aldrich

A0812

N-乙酰神经氨酸

Sigma-Aldrich

P4809

磷酸盐-柠檬酸盐缓冲液

技术

HPLC: suitable

试验参数

column: Ascentis Express OH5, 10 cm x 3.0 mm I.D., 2.7 μm particles (53769-U)
mobile phase: [A] acetonitrile; [B] water with 0.1% formic acid, pH 3.5 with ammonium formate; (75:25, A:B)
flow rate: 0.4 mL/min
pressure: 1350 ppi (93 bar)
column temp.: 25 °C
detector: MS, ESI (+), TIC, m/z 150-300
injection: 5 μL

适用性

application for HPLC

应用

chemicals and industrial polymers

分析说明

L-methionine sulfoximine and L-buthionine sulfoximine are used in are used to prevent additional enzyme activity in Chinese hampster ovary (CHO) cell lines with supplemental Glutamine Synthase (GS). It is of interest to be able to monitor both purity of such molecules in order to control addition to cell media as well as for the assay of parent molecules during use. The analytes are highly polar and thus should be amenable to HILIC-LC-MS analysis. This first installment investigates the ability of Ascentis Express HILIC and Ascentis Express OH5 stationary phases for the separation of the methionine analog and related compounds as well as methionine and buthionine separations.