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一般描述
唾液酸定量检测试剂盒提供了一种快速精确的测定总N-乙酰神经氨酸（NANA；唾液酸）的方法。NANA是通过神经氨酸酐酶从糖复合物中释放出来的。该试剂盒使用了α(2→3,6,8,9)神经氨酸酐酶来剪切所有的NANA连接，其中包括α(2→8)及α(2→9)连接，以及分支状NANA等，可对总唾液酸含量进行最精确的测定。
应用
含有定量自由的、或糖蛋白、细胞表面糖蛋白、聚唾液酸及荚膜多糖（仅由聚唾液酸组成）中的N-乙酰神经氨酸所需的所有试剂。
数量
试剂可用于进行25次反应，其中包括对照样品。每次反应可检测1-200 nmole的NANA。
仅试剂盒组分
产品编号
说明
- N-Acetylneuraminic acid aldolase 25 μL
- N-Acetylneuraminic acid 200 μL
- α(2→3,6,8,9)-Neuraminidase 25 μL
- Fetuin, bovine .5 mg
- Lactic dehydrogenase 25 μL
- β-NADH disodium salt 3 vial(s)
- Tris-HCl, pH 7.5 1 mL
- Sialidase buffer 500 μL
查看所有结果 (8)
法规信息
监管及禁止进口产品
A P Corfield et al.
Biochimica et biophysica acta, 744(2), 121-126 (1983-04-28)
The anomeric specificity of six sialidases (Vibrio cholerae, Arthrobacter ureafaciens, Clostridium perfringens, Newcastle disease virus, fowl plague virus and influenza A2 virus sialidases) was assessed with sialylated antifreeze glycoprotein, ovine submandibular gland glycoprotein and alpha 1-acid glycoprotein, resialylated specifically in
Pragasam Viswanathan et al.
Urological research, 39(4), 269-282 (2011-01-14)
Tamm-Horsfall protein (THP) is thought to protect against calcium oxalate monohydrate (COM) stone formation by inhibiting COM aggregation. Several studies reported that stone formers produce THP with reduced levels of glycosylation, particularly sialic acid levels, which leads to reduced negative
Analysis of glycoprotein-associated oligosaccharides.
R A Dwek et al.
Annual review of biochemistry, 62, 65-100 (1993-01-01)
Corina Daniela Ene et al.
Metabolites, 11(1) (2020-12-31)
Posttranslational modifications are dynamic enzymatic-mediated processes, regulated in time and space, associated with cancer development. We aimed to evaluate the significance of posttranslational modifications in the pathogenesis of clear cell renal cell carcinoma. The authors developed a prospective, observational study
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