LC/MS Analysis of Polypeptide Drugs on Ascentis^® Express RP-Amide

技术

HPLC: suitable

试验参数

column: Ascentis Express RP-Amide, 10 cm x 2.1 mm I.D., 2.7 μm particles (53913-U)
mobile phase: [A] 10 mM ammonium acetate in water, pH 4.2; [B] 10 mM ammonium acetate in 90:10 (v/v) acetonitrile:water, pH 4.7
gradient: held at 20% B for 0.1 min; 20 to 80% B in 2.9 min; 80 to 20% B in 0.01 min; held at 20% B for 0.99 min
flow rate: 0.3 mL/min
column temp.: 35 °C
detector: TOF, ESI(+), m/z 100-2500
injection: 3 μL
sample: 1 μL/mL in 10mM ammonium formate, 80:20, acetonitrile:water

适用性

application for HPLC

应用

life science and biopharma