技术
HPLC: suitable
试验参数
column: Ascentis Express RP-Amide, 10 cm x 2.1 mm I.D., 2.7 μm particles (53913-U)
mobile phase: [A] 10 mM ammonium acetate in water, pH 4.2; [B] 10 mM ammonium acetate in 90:10 (v/v) acetonitrile:water, pH 4.7
gradient: held at 20% B for 0.1 min; 20 to 80% B in 2.9 min; 80 to 20% B in 0.01 min; held at 20% B for 0.99 min
flow rate: 0.3 mL/min
column temp.: 35 °C
detector: TOF, ESI(+), m/z 100-2500
injection: 3 μL
sample: 1 μL/mL in 10mM ammonium formate, 80:20, acetonitrile:water
适用性
application for HPLC
应用
life science and biopharma
分析说明
This application demonstrates the suitability of the Ascentis Express RP Amide for the analysis of polypeptide drugs.
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
分析物
说明
Oxytocin
[deamino-Cys1, D-Arg8]-Vasopressin acetate salt hydrate
分析色谱柱
标准
流动相组分
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