HPLC Analysis of Fluoroquinolone Antibiotics on Ascentis^® Express C18

技术

HPLC: suitable

试验参数

column: Ascentis Express C18, 10 cm x 3.0 mm I.D., 2.7 μm particles (53814-U)
mobile phase: [A] 0.1% formic acid in water; [B] 0.1% formic acid in acetonitrile; (85:15, A:B)
flow rate: 1.2 mL/min
column temp.: 35 °C
detector: UV, 254 nm
injection: 4 μL
sample: 200 ng/mL in 25:75, water: acetonitrile

适用性

application for HPLC

应用

food and beverages
pharmaceutical (small molecule)