技术
HPLC: suitable
试验参数
column: Ascentis Express Phenyl-Hexyl, 5 cm x 4.6 mm I.D., 2.7 μm particles (53348-U)
mobile phase: [A] water; [B] acetonitrile; (62:38, A:B)
flow rate: 1.4 mL/min
pressure: 2600 psi (179 bar)
column temp.: 35 °C
detector: UV, 250 nm
injection: 2 μL
sample: 0.1 g/mL of each in 20% methanol
适用性
application for HPLC
应用
clinical
pharmaceutical (small molecule)
分析说明
The Phenyl-Hexyl phase of the Ascentis Express product line displays the best selectivity of this sample; selectivity is markedly affected by choice of organic modifier. The application also demonstrates feasibility of fast LC with Ascentis Express by virtue of low backpressure and high efficiency.
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
分析物
说明
Oxazepam
Alprazolam
Clonazepam
Desmethyldiazepam
Diazepam
分析色谱柱
标准
产品编号
说明
价格
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