HPLC Analysis of Drugs of Abuse on Ascentis^® Express F5

技术

HPLC: suitable

试验参数

column: Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U)
mobile phase: [A] 5 mM ammonium formate; [B] 5 mM ammonium formate in 95:5 (v/v) acetonitrile:water
gradient: held at 20% B for 0.5 min; 20 to 100% B in 5.5 min; held at 100% B for 4 min
flow rate: 0.3 mL/min
pressure: 175 bar
column temp.: 35 °C
detector: ESI(+), m/z 100-1000
injection: 1 μL
sample: 1 μg/mL in 50:50, water: methanol

适用性

application for HPLC

应用

forensics and toxicology