HPLC Analysis of Tricyclic Antidepressants on Ascentis^® ES Cyano

技术

HPLC: suitable

试验参数

column: Ascentis ES Cyano, 15 cm x 4.6 mm I.D., 5 μm particles (577306-U)
mobile phase: [A] 10 mM potassium phosphate dibasic, pH 7 with phosphoric acid; [B] acetonitrile; [C] methanol; (25:60:15, A:B:C)
flow rate: 2 mL/min
column temp.: 25 °C
detector: UV, 215 nm
injection: 5 μL
sample: 100 μg/mL in water: methanol (70:30)

适用性

application for HPLC

应用

pharmaceutical (small molecule)