HPLC Analysis of Luciferin Enantiomers on Astec^® CHIROBIOTIC^® V

技术

HPLC: suitable

试验参数

column: CHIROBIOTIC V, 25 cm x 4.6 mm I.D., 5 μm particles (11024AST)
mobile phase: [A] 20 mM ammonium nitrate, pH 5.5; [B] THF; (90:10, A:B)
flow rate: 1 mL/min
column temp.: ambient
detector: UV, 254 nm
injection: 6 μL
sample: 0.5 mg/mL in methanol

适用性

application for HPLC

应用

chemicals and industrial polymers
life science and biopharma