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Merck
CN

Y1250

Sigma-Aldrich

酵母含氨基酸的氮碱

Non-selective yeast growth medium

别名:

ynb with amino acids

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About This Item

UNSPSC代码:
41106212
NACRES:
NA.85

等级

for molecular biology

质量水平

无菌性

non-sterile

形式

powder

技术

microbiological culture: suitable

pH值(酸碱度)

5.3-5.5(0.67% solution)

应用

microbiology

储存温度

room temp

适用性

nonselective for Candida spp.
nonselective for Pichia spp.
nonselective for Saccharomyces spp.
nonselective for Zygosaccharomyces spp.

一般描述

酵母氮源是一种高参考生长培养基,用于培养酵母。这种富含营养的微生物肉汤含有氨基酸、氮、维生素、微量元素和盐类。

应用

含氨基酸酵母氮源已用于:
  • 大肠杆菌的ZYP-31介质制备
  • 合成葡萄糖(SD)介质,用于评估氮源效果
  • 培养产朊假丝酵母,生产作为内标物的13C标记酵母提取物
  • 成像培养基制备

适用于根据碳需要对酵母菌进行分类。也适用于酵母的普通生长,需要添加碳源(通常为葡萄糖)。含氨基酸酵母氮源是普通生长介质的良好氮源。包含酵母生长必需的营养素,不含碳水化合物。

组分

成分、含量(& # 956克/升,除非另有说明)

氮源:
硫酸铵,5.0 g/L

氨基酸:
L-组氨酸,10 mg/L
DL-蛋氨酸,20 mg/L
DL-色氨酸,20 mg/L

维生素:
生物素,2.0
泛酸钙,400
叶酸,2.0
肌醇,2.0 mg/L
烟酸,400
对氨基苯甲酸,200
盐酸吡哆醇,400
核黄素,200
盐酸硫胺,400

微量元素:
硼酸
硫酸铜
碘化钾
氯化铁
硫酸镁
钼酸钠
硫酸锌

盐类:
磷酸二氢钾,1.0 g/L
硫酸镁,0.5 g/L
氯化钠,0.1 g/L
氯化钙,0.1 g/L

重悬

搅拌以使6.7g酵母氮源基础粉末和5 g葡萄糖悬浮在100 mL水中,制成10倍浓缩液。必要时加热以帮助溶解。过滤消毒,并2-8℃条件下储存。

向900mL无菌水中加入100mL浓缩液,将10倍浓缩液稀释成1倍工作溶液。

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

动植物来源培养基

分析证书(COA)

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G U Balcke et al.
Toxicology letters, 203(3), 200-209 (2011-03-16)
The study presented here describes the application of metabolite profiling of highly polar, intracellular metabolites after incubation of a mammalian fibroblast cell line with inhibitors of mitochondrial function. A metabolomics approach was used to assess the complex response of the
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Ude C, et al.
Sensors and Actuators B, Chemical, 221, 1035-1043 (2015)
Corinne Maufrais et al.
G3 (Bethesda, Md.) (2021-04-07)
The genomes of a large number of Cryptococcus neoformans isolates have been sequenced and analyzed in recent years. These genomes have been used to understand the global population structure of this opportunistic pathogen. However, only a small number of South
Live bio-imaging with fully bio-compatible organic fluorophores
Yang I, et al.
Journal of Photochemistry and Photobiology. B, Biology, 166, 52-57 (2017)
Renée Hermanns et al.
Journal of fungi (Basel, Switzerland), 5(3) (2019-09-12)
Recurrent vulvovaginal candidiasis (RVVC) is predicted to increase to almost 158 million cases annually by 2030. Extensive self-diagnosis and easily accessible over-the-counter (OTC) fungistatic drugs contribute to antifungal-resistance, illustrating the need for novel therapies. Honey possesses multiple antimicrobial mechanisms, and

实验方案

酵母因能够快速生长并具有分散的细胞而被认为是用于真核生物研究的模范系统。酵母培养物可采用类似于细菌培养物的技术在液体培养基或琼脂平板上进行生长、维持和保存。

Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.

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