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安全信息

T5574

Sigma-Aldrich

TB培养基

Liquid microbial growth medium

别名:

TB 培养基, Tartoff – Hobbs 培养基

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About This Item

UNSPSC代码:
41106200
NACRES:
NA.85

等级

for molecular biology

质量水平

500

描述

Liquid Medium

无菌性

sterile; 0.2 μm filtered

表单

liquid

技术

microbiological culture: suitable

应用

microbiology

储存温度

room temp

适用性

nonselective for Escherichia coli
nonselective for coliforms

相关类别

一般描述

Terrific Broth是一个被高度引用的用于大肠杆菌培养的微生物生长培养基。 这种营养丰富的微生物肉汤含有甘油、肽、氨基酸、水溶性维生素和碳水化合物。

应用

Terrific肉汤用于非选择性培养大肠杆菌菌株，用于克隆、高产量生产DNA质粒和高产量生产重组蛋白。引用 它也适用于添加适当抗生素时的选择性培养。

特点和优势

Terrific Broth液可提供：
  • 过滤器灭菌，即用型
  • 方便包装规格
  • 标准配方

组分

23.6g/L酵母提取物
11.8g/L 胰蛋白胨
9.4g/L K2HPO4
2.2g/L KH2PO4
4ml/L甘油

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves

法规信息

动植物来源培养基

从最新的版本中选择一种：

分析证书(COA)

Lot/Batch Number
SLCN1739
SLCH8540
SLCG5460
SLCF9918
SLCC9230

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如果您需要特殊版本，可通过批号或批次号查找具体证书。

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在文件库中查找您最近购买产品的文档。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Which bacterial culture medium is the best choice for my application?

    Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production.  The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Shaheen Shojania et al.
Journal of virological methods, 164(1-2), 35-42 (2009-11-28)
The HIV-1 transactivator of transcription (Tat) is a protein essential for virus replication. Tat is an intrinsically disordered RNA-binding protein that, in cooperation with host cell factors cyclin T1 and cyclin-dependent kinase 9, regulates transcription at the level of elongation.
The preparation and analysis of DNA for use in transgenic technology.
Auerbach, A.B., et al.
Advanced Protocols For Animal Transgenesis, 501-501 (2011)
Andrey Sokolov et al.
Proceedings of the National Academy of Sciences of the United States of America, 107(3), 969-974 (2010-01-19)
Whereas the laws of thermodynamics prohibit extraction of useful work from the Brownian motion of particles in equilibrium, these motions can be "rectified" under nonequilibrium conditions, for example, in the presence of asymmetric geometrical obstacles. Here, we describe a class
Shani Leviatan et al.
The Journal of biological chemistry, 285(31), 23548-23556 (2010-06-08)
Membrane proteins constitute 20-30% of all proteins encoded by the genome of various organisms. Large amounts of purified proteins are required for activity and crystallization attempts. Thus, there is an unmet need for a heterologous membrane protein overexpression system for
Ørjan Samuelsen et al.
Antimicrobial agents and chemotherapy, 52(8), 2905-2908 (2008-06-19)
Purified recombinant VIM-7 possesses efficient penicillinase and carbapenemase activities comparable to those of VIM-2. Cephalosporinase activity was variable and generally lower than those of VIM-1 and VIM-2. A homology model suggests that the VIM-7 Tyr-218 Phe substitution may be responsible
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实验方案

Plasmid DNA Preparation

Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

微生物生长实验方案

在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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