等级
for molecular biology
质量水平
描述
Miller′s Modification, 10 g/L NaCl
无菌性
non-sterile
形式
powder
组成
NaCl, 10 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L
pH值（酸碱度）
6.8-7.2(2.5% solution)
应用
food and beverages
储存温度
room temp
适用性
nonselective for Escherichia coli
nonselective for coliforms
一般描述
应用
适用于大肠杆菌菌株的非选择性培养，用于克隆、DNA质粒生产和重组蛋白生产。当加入适当的抗生素时，也适合选择性培养。
生化/生理作用
特点和优势
- 包装尺寸更大，易于扩大规模
- 液体的廉价替代品
- 标准配方
制备说明
2.121 °C下高压灭菌15分钟。
制备Luria、Adams和Ting培养基（也称为LC液体培养基）：高压灭菌后，无菌加入25 mL无菌0.1M氯化钙。
重悬
相关产品
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What are the differences among the Luria, Lennox and Miller LB formulations?
LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose. Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version). For some applications, even lower salt is required (Luria's low salt version).
What is the difference among the LB - Miller products?
LB - Miller is available in many types to suit your needs. The different product formats include powder and liquid form. The powder form is also available with agar for easy LB-agar plate preparation.L2542 (LB Miller liquid)L3522 (LB Miller powder)L3147 (LB Miller powder with agar)
Which bacterial culture medium is the best choice for my application?
Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production. The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.
Will adding magnesium to the culture medium increase cell density?
In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities. You may need to also increase the shaking speed of the incubator.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
商品
Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.
基因工程和克隆的发展为研究领域开辟了表达和分离异源蛋白质的许多可能性。技术上巨大进步使得能够大规模表达和分离重组蛋白。
SeqPlex™-I WTA kit amplifies RNA for NGS, enabling genomic studies from limited samples.
实验方案
General protocols for growth of competent cells in microbial medium.
在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。
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