SAB4200766
Anti-Nitric Oxide Synthase, Inducible antibody, Mouse monoclonal
clone NOS-IN, purified from hybridoma cell culture
别名:
Anti-Hepatocyte NOS (HEP-NOS), Anti-Inducible NOS (iNOS), Anti-NOS type II, Anti-Peptidyl-cysteine S-nitrosylase NOS2
产品名称
Anti-Nitric Oxide Synthase, Inducible antibody, Mouse monoclonal, clone NOS-IN, purified from hybridoma cell culture
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
NOS-IN, monoclonal
form
buffered aqueous solution
mol wt
~130 kDa
species reactivity
human, mouse, rat, plant
concentration
~1.0 mg/mL
technique(s)
flow cytometry: suitable
immunoblotting: 2-4 μg/mL using mouse macrophage RAW 264.7 cell line activated with lipopolysaccharide (LPS) and interferon-γ
immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human pancreas sections
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
mouse ... Nos2(18126)
Application
Anti-Nitric Oxide Synthase, Inducible antibody, Mouse monoclonal may be used:
- immunoblotting
- immunofluorescence
- immunohistochemistry
- fluorescence-activated cell sorting (FACS)
Biochem/physiol Actions
Anti-Nitric Oxide Synthase, Inducible antibody, Mouse monoclonal reacts specifically with inducible-type nitric oxide synthase (iNOS, also termed macNOS and mNOS). It does not react with NOS derived from brain (bNOS) or with endothelial-type NOS (eNOS). The antibody recognizes iNOS from human, mouse, rat and plant origin.
Inducible nitric oxide synthase (iNOS) or NOS2 catalyzes the oxidation of L-arginine to L-citrulline and nitric oxide (NO) via an OH-L-arginine intermediate in the presence of reduced nicotinamide adenine dinucleotide phosphate (NADPH) and oxygen. Elevated levels of iNOS is observed in asthma. A high level of iNOS leads to an increase in NO production which may contribute to cell death and tissue damage. It is also implicated in the pathophysiology of septic shock. Single nucleotide polymorphism in the iNOS gene is indicative of high risk for B and T-cell non-Hodgkin lymphoma. iNOS also elicits macrophage bactericidal functionality.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Anti-Nitric Oxide Synthase, Inducible antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the NOS-IN hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse. Inducible-type nitric oxide synthase (NOS) of mouse macrophage origin (iNOS) or macrophage NOS (mNOS) is a type II NOS (NOS2). It is a Ca2+-independent protein, expressed in activated macrophages and some glial cells after stimulation. NOS2 is also present in several other cell types including hepatocytes, chondrocytes, endothelial cells and fibroblasts. The iNOS gene is mapped to human chromosome 17q11.2. iNOS is a dimeric flavoprotein with a heme group. It comprises the C-terminal reductase domain and an N-terminal oxygenase domain.
Immunogen
synthetic peptide corresponding to the C terminal region of nitric oxide synthase (NOS) of mouse macrophage origin (iNOS, also termed macNOS), conjugated to KLH
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Cleva Villanueva et al.
Free radical biology & medicine, 49(3), 307-316 (2010-04-15)
The effects of nitric oxide in biological systems depend on its steady-state concentration and where it is being produced. The organ where nitric oxide is produced is relevant, and within the organ, which types of cells are actually contributing to
Christian Bogdan
Trends in immunology, 36(3), 161-178 (2015-02-18)
Thirty years after the discovery of its production by activated macrophages, our appreciation of the diverse roles of nitric oxide (NO) continues to grow. Recent findings have not only expanded our understanding of the mechanisms controlling the expression of NO
iNOS (NOS2) at a glance
Lowenstein CJ and Padalko E
Journal of Cell Science, 117(14), 2865-2867 (2004)
Antonio Contestabile et al.
Current neuropharmacology, 10(4), 303-310 (2013-06-05)
Nitric oxide (NO) is a versatile cellular messenger performing a variety of physiologic and pathologic actions in most tissues. It is particularly important in the nervous system, where it is involved in multiple functions, as well as in neuropathology, when
Federica Vannini et al.
Redox biology, 6, 334-343 (2015-09-04)
Nitric oxide (NO) is one of the 10 smallest molecules found in nature. It is a simple gaseous free radical whose predominant functions is that of a messenger through cGMP. In mammals, NO is synthesized by the enzyme nitric oxide
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