N-CMV-30 Sequencing Primer

The N-CMV-30 sequencing primer is a chemically synthesized, 30 base, single strand oligodeoxyribonucleotide designed for dsDNA or ssDNA sequencing of FLAG^® fusion proteins at the C-terminus of the pFLAG-CMV expression vectors. The primer is complementary to map position nucleotides 818-847 of positive, coding DNA strand of the pFLAG-CMV vectors.

The N-CMV-30 sequencing primer is suitable for sequencing from the C-terminus of pFLAG-CMV expression vectors.

The primer is supplied at an initial concentration of 5 uM in 1xTE buffer. The nucleotide sequence is as follows: 5′-AAT-GTC-GTA-ATA-ACC-CCG-CCC-CGT-TGA-CGC-3′

(5′-AAT GTC GTA ATA ACC CCG CCC CGT TGA CGC-3′)

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany