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Merck
CN

10814270001

Roche

cDNA合成引物

lyophilized, pkg of 40 μg

别名:

oligo-p(dt)15

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About This Item

UNSPSC代码:
41106300

价格与库存信息目前不能提供

表单

lyophilized

质量水平

包装

pkg of 40 μg

制造商/商品名称

Roche

储存温度

−20°C (−15°C to −25°C)

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此商品
05038A668656763
Agar suitable for microbiology

05038

Agar

Agar Bacteriological, microbiology tested, suitable for plant cell culture, granular

A6686

Agar

Agar high purity, low ionic content, low gel strenght, suitable for microbiology

56763

Agar

form

powder

form

powder

form

granular

form

powder

grade

Molecular Biology

grade

-

grade

-

grade

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

一般描述

该引物由15聚体寡核苷酸组成,该寡核苷酸在每个位置均有胸苷(T)。 该寡核苷酸通过 HPLC 纯化、脱盐,并以锂盐冻干粉的形式提供。 p(dT)15 引物的 5′ 末端没有进行磷酸化。Oligo(dT)N 生成全长cDNA。如果模板含有在内部拉伸的oligo(A),则引物可能会与之结合,从而导致引导错误(mis priming)和非全长的转录本。

组成: p(dT)15[ 15聚体寡核苷酸,只含脱氧胸苷(dT)残基].

磷酸化: p(dT)15引物的 5′ 末端没有进行磷酸化。

应用

cDNA合成引物可用于:
  • RT-PCR[1][2][3]
  • cDNA文库的生成
  • 第一链cDNA的合成

单位定义

8 nmol ξ 1 A260 unit

其他说明

仅用于生命科学研究。不可用于诊断。

储存分类代码

12 - Non Combustible Liquids

WGK

nwg

闪点(°F)

does not flash

闪点(°C)

does not flash


历史批次信息供参考:

分析证书(COA)

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Khalil Farhat et al.
Frontiers in immunology, 9, 2175-2175 (2018-10-20)
The precise impact of the somatotrope axis upon the immune system is still highly debated. We have previously shown that mice with generalized ablation of growth hormone (GH) releasing hormone (GHRH) gene (Ghrh-/-) have normal thymus and T-cell development, but
Agnes Bonnet et al.
BMC genomics, 12, 417-417 (2011-08-20)
Successful achievement of early folliculogenesis is crucial for female reproductive function. The process is finely regulated by cell-cell interactions and by the coordinated expression of genes in both the oocyte and in granulosa cells. Despite many studies, little is known
Bingshe Han et al.
PloS one, 7(5), e36941-e36941 (2012-06-01)
Our previous study shows that inhibiting activator protein one (AP1) transcription factor function in murine epidermis, using dominant-negative c-jun (TAM67), increases cell proliferation and delays differentiation. To understand the mechanism of action, we compare TAM67 impact in mouse epidermis and

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