P1261
磷酸化酶 a 来源于兔肌肉
lyophilized powder, 20-30 units/mg protein
别名:
1,4-α-D-葡聚糖：正磷酸盐 α-D葡萄糖基转移酶
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-878-8
MDL number:
Specific activity:
20-30 units/mg protein
Biological source:
rabbit muscle
biological source
rabbit muscle
Quality Level
form
lyophilized powder
specific activity
20-30 units/mg protein
purified by
2× crystallization
composition
Protein, 30-60% E1%/280
foreign activity
AMP-phosphatase, ATP-phosphatase, debrancher enzyme and phosphorylase phosphatase ≤0.1%, phosphoglucomutase ≤0.25%, phosphorylase b ≤30%
storage temp.
−20°C
General description
磷酸化酶 A 是糖原磷酸化酶的活性形式，其将糖原和正磷酸盐（Pi）转化为葡萄糖1-磷酸（G-1-P）。
Application
来自兔肌肉的磷酸化酶已用于混合糖原以制备极限糊精。它还被用于探测寡聚糖微阵列磷酸化酶活性分析中的阵列。
来自兔肌肉的磷酸化酶已被用于研究中评估齐墩果酸的分子机制。它也被用于研究中描述在 32P-ATP 存在下磷酸化酶 B 通过转化酶催化转化为磷酸化酶 A 的过程。
Biochem/physiol Actions
磷酸化酶参与糖原分解。磷酸化酶缺乏导致多种糖原贮积病和肝肿大。
磷酸化酶 A 可以被以下化合物抑制：多氯联苯、多氯联苯和多溴联苯。
通过磷酸化酶激酶的作用，二聚磷酸化酶 b 被转化为更有活性的四聚体磷酸化酶 a。
Physical form
冻干粉末，含有 β-甘油磷酸盐和 EDTA
Other Notes
在含有磷酸葡萄糖苷酶，NADP 和葡萄糖-6-磷酸脱氢酶的系统中测量，一个单位的酶在 pH 6.8，30℃ 下每分钟将糖原和正磷酸盐转化为 1.0 μmole α-D-1 葡萄糖 1-磷酸酯。（一个 μmolar 单位相当于约 45 Cori 单位。）
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
Activity of porcine muscle glycogen debranching enzyme in relation to pH and temperature
Kyla-Puhju M, et al.
Meat Science, 69(1), 143-149 (2005)
Hypoglycemia in the toddler and child
Pediatric Endocrinology, Diabetes, and Metabolism, 920-955 (2014)
Ian J Tetlow et al.
The Plant cell, 16(3), 694-708 (2004-02-20)
Protein phosphorylation in amyloplasts and chloroplasts of Triticum aestivum (wheat) was investigated after the incubation of intact plastids with gamma-(32)P-ATP. Among the soluble phosphoproteins detected in plastids, three forms of starch branching enzyme (SBE) were phosphorylated in amyloplasts (SBEI, SBEIIa
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| P1261-10MG | 04061834354307 |
| P1261-25MG | 04061834354314 |