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安全信息

P1261

Sigma-Aldrich

磷酸化酶 a 来源于兔肌肉

lyophilized powder, 20-30 units/mg protein

别名:

1,4-α-D-葡聚糖：正磷酸盐 α-D葡萄糖基转移酶

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About This Item

CAS号:
9032-10-4
EC 号:
2.4.1.1 (BRENDA, IUBMB)
EC 号:
232-878-8
MDL编号:
MFCD00167428
UNSPSC代码:
12352204
NACRES:
NA.54

生物来源

rabbit muscle

质量水平

200

形式

lyophilized powder

比活

20-30 units/mg protein

纯化方式

2× crystallization

组成

Protein, 30-60% E1%/280

异质活性

AMP-phosphatase, ATP-phosphatase, debrancher enzyme and phosphorylase phosphatase ≤0.1%
phosphoglucomutase ≤0.25%
phosphorylase b ≤30%

储存温度

−20°C

一般描述

磷酸化酶 A 是糖原磷酸化酶的活性形式，其将糖原和正磷酸盐（Pi）转化为葡萄糖1-磷酸（G-1-P）。

应用

来自兔肌肉的磷酸化酶已用于混合糖原以制备极限糊精。它还被用于探测寡聚糖微阵列磷酸化酶活性分析中的阵列。
来自兔肌肉的磷酸化酶已被用于研究中评估齐墩果酸的分子机制。它也被用于研究中描述在 32P-ATP 存在下磷酸化酶 B 通过转化酶催化转化为磷酸化酶 A 的过程。

生化/生理作用

磷酸化酶 A 可以被以下化合物抑制：多氯联苯、多氯联苯和多溴联苯。
磷酸化酶参与糖原分解。磷酸化酶缺乏导致多种糖原贮积病和肝肿大。
通过磷酸化酶激酶的作用，二聚磷酸化酶 b 被转化为更有活性的四聚体磷酸化酶 a。

单位定义

在含有磷酸葡萄糖苷酶，NADP 和葡萄糖-6-磷酸脱氢酶的系统中测量，一个单位的酶在 pH 6.8，30℃ 下每分钟将糖原和正磷酸盐转化为 1.0 μmole α-D-1 葡萄糖 1-磷酸酯。（一个 μmolar 单位相当于约 45 Cori 单位。）

外形

冻干粉末，含有 β-甘油磷酸盐和 EDTA

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

动植物源性产品

分析证书(COA)

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. How is Phosphorylase A, Product P1261, prepared?

    To prepare Phosphorylase A (P1261), Sigma uses a method similar to the one described in the J. Biol.Chem., 231, 65 (1958).

  6. What is the final buffer Phosphorylase A, Product P1261, is in prior to lyophilization?

    The final buffer Phosphorylase A (P1261) is in prior to lyophilization is 0.1MB-glycerophosphate and 4 mM EDTA.

  7. What is the solubility of Phosphorylase A, Product P1261?

    Sigma tests solubility of Phosphorylase A (P1261) in water at a concentration of 1 mg/ml.

  8. What is the molecular weight of Phosphorylase A, Product P1261?

    The molecular weight of Rabbit Phosphorylase A (tetramer) is 370,000 per Biochemistry 6 (1967) p. 3315 (which was determined by sedimentation and "experiments in a variety of buffers, temperatures, and ionic strength").  The molecular weight by sequence analysis (Proc. Natl. Acad. Sci. USA, 74, 4762-4766) is 97,412.

  9. What is the pI of Phosphorylase A, Product P1261?

    The pI of Phosphorylase A (P1261) is 5.6 - 5.8.

  10. Has the amino acid sequence of phosphorylase from rabbit muscle been published?

    The sequence has been published. The amino acid composition is:Asp5l, Asn45, Thr35, Ser29, Glu64, Gln31, Pro36, Gly48, Ala63, Cys9, Val62, Met2l, Ile49, Leu79, Tyr36, Phe38, Trp12, Lys48, HiS22, and Arg63. Reference for the sequence and amino acid composition: K. Titani et al., Proc. Nat. Acad. Sci., USA, 74, 4762-4766 (1977) (see attached pdf).Product P1261 is phosphorylase a, which is the tetrameric form. Products P4649 and P6635 are phosphorylase b, the dimeric  form. The sequence information of the subunit would be the same for both forms.

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Glycogen Phosphorylase Structures and Function
Fletterick, R. and S. Sprang
Accounts of Chemical Research, 15, 361-369 (1982)
Ian J Tetlow et al.
The Plant cell, 16(3), 694-708 (2004-02-20)
Protein phosphorylation in amyloplasts and chloroplasts of Triticum aestivum (wheat) was investigated after the incubation of intact plastids with gamma-(32)P-ATP. Among the soluble phosphoproteins detected in plastids, three forms of starch branching enzyme (SBE) were phosphorylated in amyloplasts (SBEI, SBEIIa
R C Mead et al.
Biochimica et biophysica acta, 701(2), 173-179 (1982-02-18)
Polychlorinated biphenyls, polychlorinated biphenylols and polybrominated biphenyls inhibited both rabbit muscle phosphorylase a and phosphorylase b (1,4-alpha-D-glucan:orthophosphate alpha-d-glucosyltransferase, EC 2.4.1.1). The degree of inhibition was dependent upon the relative hydrophobicity of the compounds and steric hinderance. 2,4,5,2',4',5'-Hexabromobiphenyl and Firemaster BP-6
Activity of porcine muscle glycogen debranching enzyme in relation to pH and temperature
Kyla-Puhju M, et al.
Meat Science, 69(1), 143-149 (2005)
Hypoglycemia in the toddler and child
Pediatric Endocrinology, Diabetes, and Metabolism, 920-955 (2014)
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