P3397
磷酸葡萄糖变位酶 来源于兔肌肉
ammonium sulfate suspension, ≥100 units/mg protein
别名:
PGM, α-D-葡萄糖-1,6-二磷酸酶, α-D-葡萄糖磷酸转移酶
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥100 units/mg protein
Biological source:
rabbit muscle
biological source
rabbit muscle
description
ammonium sulfate suspension, >=100 units/mg protein
form
ammonium sulfate suspension
specific activity
≥100 units/mg protein
storage condition
(Tightly closed)
technique(s)
activity assay: suitable
color
white to light yellow
foreign activity
lactic dehydrogenase ≤0.5%, phosphoglucose isomerase ≤0.01%, pyruvate kinase ≤0.05%
shipped in
wet ice
storage temp.
2-8°C
Quality Level
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General description
适合研究领域：细胞信号传导
磷酸葡萄糖变位酶(PGM)，是一种保守酶，在动物、植物和微生物中大量存在，几乎分布于所有组织中。
磷酸葡萄糖变位酶(PGM)，是一种保守酶，在动物、植物和微生物中大量存在，几乎分布于所有组织中。
Application
本品（磷酸葡萄糖变位酶）已用于：
- 研究人类的糖原合成与磷酸葡萄糖变位酶缺乏
- 通过检测磷酸葡萄糖变位酶的活性研究代谢调控
- 试验A（参见文献），检测BL21(DE3)C43转化细胞中的糖原代谢密切相关酶
- 蔗糖合酶(SuSy)和ADP葡萄糖焦磷酸化酶(AGPase)检测(3)
- 在酶活性测定中结合烟酰胺腺嘌呤二核苷酸磷酸(NADP+)还原反应，测定来自蔗糖和无机磷酸(Pi)的葡萄糖-1-磷酸
Biochem/physiol Actions
磷酸葡萄糖变位酶(PGM)在糖酵解和糖异生中起着至关重要的作用。参与糖原和昆虫海藻糖的代谢。磷酸葡萄糖变位酶也参与植物和某些微生物的发育。葡萄糖-6-磷酸是核心代谢产物，因此PGM是蛋白质、脂质和核酸的代谢关键，对植物发育至关重要。磷酸葡萄糖变位酶催化葡萄糖-6-磷酸(G-6-P)和葡萄糖-1-磷酸(G-1-P)的相互转变。
Physical form
(NH4)2SO4，pH 6.0，含 0.01%EDTA 的晶体混悬液
Analysis Note
缩二脲法测定蛋白
Other Notes
1 个单位将在 30°C、pH 7.4 下每分钟将 1.0 μmolα-D-葡萄糖 1-磷酸转化为 α-D-葡萄糖 6-磷酸。
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
低风险生物材料
此项目有
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The Journal of biological chemistry, 262(35), 17038-17045 (1987-12-15)
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Bi-Ying Pan et al.
3 Biotech, 10(2), 61-61 (2020-02-08)
Phosphoglucomutase (PGM) is a key enzyme in glycolysis and gluconeogenesis, regulating both glycogen and trehalose metabolism in insects. In this study, we explored the potential function of phosphoglucomutase (PGM) using RNA interference technology in Nilaparvata lugens, the brown planthopper. PGM1
商品
Instructions for working with enzymes supplied as ammonium sulfate suspensions
以硫酸铵悬浮液形式提供的酶的使用指南
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