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安全信息

L6876

Sigma-Aldrich

Lysozyme 来源于鸡蛋白

powder or granules, ≥39,000 units/mg protein

别名:

粘肽N-乙酰胞壁质水解酶, 胞壁质酶

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About This Item

CAS号:
12650-88-3
EC 号:
3.2.1.17 (BRENDA, IUBMB)
EC 号:
235-747-3
MDL编号:
MFCD00131557
UNSPSC代码:
12352204
NACRES:
NA.54

生物来源

chicken egg white

质量水平

200
300

表单

powder or granules

比活

≥39,000 units/mg protein

分子量

single-chain 14.3 kDa

技术

cell based assay: suitable

适用性

suitable for cell lysis

UniProt登记号

P00698

应用

cell analysis

运输

wet ice

储存温度

room temp

基因信息

chicken ... LYZ(396218)

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相关类别

应用

来自鸡蛋清的溶菌酶已用于从细菌细胞中提取基因组DNA。它已被用作MALDI-TOF（基质辅助激光解吸电离-飞行时间）质谱分析的一种外部标准品。
酶可分解细菌的细胞壁；用于制备原生质体。

生化/生理作用

溶菌酶可水解肽聚糖中N-乙酰胞壁酸与N-乙酰-D-氨基葡萄糖残基之间以及壳糊精中N-乙酰-D-氨基葡萄糖残基之间的β（1→4）连接。 革兰氏阳性细胞对这种水解非常敏感，因为它们的细胞壁中的肽聚糖比例很高。革兰氏阴性菌就对这种水解没那么敏感，因为它们有外膜并且肽聚糖的比例较低。然而，这些细胞可能在细胞外膜中螯合金属离子的EDTA存在的情况下水解。

该酶在广泛的pH范围（6.0至9.0）内具有活性。pH值为6.2时，可在更广的离子强度范围（0.02至0.100 M）观察到最大活性，而pH值为9.2时为0.01至0.06 M。

特点和优势

Lysozyme 是一种多功能酶，具有独特性质， 适用于各种应用，包括细菌细胞壁 分解、基因组DNA提取和质量分析的外部标准。 它在广泛的pH值范围内性能可靠、活跃，可采用 各种包装尺寸。

单位定义

一个单位的酶在以微球菌的悬浮液作为底物时，在 pH 6.24，25°C的条件下，在2.6 mL的反应混合液（1 cm光路）中每分钟可引起0.001的ΔA450。

其他说明

查看更多关于 复合糖分析用酶的信息， 请访问 www.sigma-aldrich.com/enzymeexplorer

象形图

Health hazard
GHS08

警示用语：

Danger

危险声明

H334

预防措施声明

P261 - P284 - P501

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

动植物源性产品

从最新的版本中选择一种：

分析证书(COA)

Lot/Batch Number
0000412786
0000412786
0000410641
0000410640
0000410643

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  1. How can Product L6876, Lysozyme from chicken egg white, be reconstituted and can stock solutions be prepared?

    For the lysis procedure, use a freshly prepared solution of 500,000 units/ml in 10 mM Tris-HCl, pH 8.0. The product is also soluble in water (10 mg/ml) yielding a clear to slightly hazy colorless solution. Aqueous solutions should be stable for at least one month when stored between 2-8 °C.

  2. Can Product L6876, Lysozyme from chicken egg white, be inactivated by heat?

    Solutions of Lysozyme may be inactivated by boiling for 40 seconds at 100°C at an alkaline pH.

  3. What is the amino acid sequence of Product L6876, Lysozyme from chicken egg white?

    Lysozyme is a single chain polypeptide of 129 amino acids. The amino acid sequence of chicken egg white Lysozyme is described in the Journal of Biological Chemistry, 238, 2698-2707 (1963).

  4. Under what conditions can Product L6876, Lysozyme from chicken egg white, be used to lyse E. coli?

    The following procedure is for the lysis of E. coli. It may be used as a guideline for other species.1. Incubate E. coli (strain ATCC 37017) bearing the pBR322 plasmid overnight in Terrific Broth (Product No. T 0918) with 25 μg/ml tetracycline (Product No T 3383) and 25 μg/ml ampicillin (Product No. A 9518).2. Centrifuge 1-2 ml samples of the overnight culture.3. Resuspend the pellets in 350 μl of STET buffer (10 mM Tris-HCl, pH 8.0, with 0.1 M NaCl, 1 mM EDTA, and 5% [w/v] TRITON® X-100).4. Add 25 μl of a freshly prepared lysozyme solution (500,000 units/ml in 10 mM Tris-HCl, pH 8.0).5. Mix by vortexing for 3 seconds.6. Incubate the lysis mixture for 30 minutes at 37 °C7. After incubation, place the tube containing the lysis mixture in a boiling water bath for exactly 40 seconds.8. Centrifuge the lysis mixture at 14,000 x g.9. Remove the pellet (cell debris) from the tube using a sterile toothpick.10. Plasmid DNA from the supernatant may then be purified and analyzed.

  5. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  6. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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商品

HPLC Analysis of Protein Standards (1) on BIOshell™ A400 Protein C4

For use as a marker in SDS-PAGE; Albumin from chicken egg white, For use as a marker in SDS-PAGE; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein

实验方案

Enzymatic Assay of Lysozyme (EC 3.2.1.17)

This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.

溶菌酶的酶学测定（EC 3.2.1.17）

该酶速率测定可用于溶菌酶产物。它不适用于在测定在琼脂糖上的重组型或不溶性溶菌酶。

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Chromatograms

application for HPLC

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