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表单
lyophilized powder
比活
≥60 units/mg protein
组成
Protein, 20-40% biuret
异质活性
ATPase ≤0.5%
phosphorylase a ≤1%
phosphorylase b ≤5%
储存温度
−20°C
一般描述
磷酸化酶激酶包含四个亚基，每个亚基含有α、β、γ以及sigma成分。该sigma成分结合4个钙分子，称为钙调蛋白，而其γ单位可用作催化亚基。
应用
来自兔肌肉的磷酸化酶激酶已应用于一项研究，以评估糖原磷酸化酶的特征。 此外，它还应用于研究肌动蛋白对不同形式肌磷酸化酶激酶的活化作用。
单位定义
在30°C、pH 7.7和ATP存在的条件下，一个单位每分钟可将磷酸化酶B转化为1.0 μ摩尔磷酸化酶。
外形
含有（NH4）2SO4、蔗糖、β-甘油磷酸盐和二硫赤藓糖醇的冻干粉
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Eijiro Ozawa
Proceedings of the Japan Academy. Series B, Physical and biological sciences, 87(8), 486-508 (2011-10-12)
It had long been one of the crucial questions in muscle physiology how glycogenolysis is regulated in connection with muscle contraction, when we found the answer to this question in the last half of the 1960s. By that time, the
G V Silonova et al.
Biokhimiia (Moscow, Russia), 49(1), 127-135 (1984-01-01)
The activation of different forms of muscle phosphorylase kinase by actin has been studied. F-actin which is polymerized by 2 mM MgCl2 is a more effective activator of phosphorylase kinase than F-actin polymerized by 50 mM KCl. There is evidence
Laura A Lane et al.
Molecular & cellular proteomics : MCP, 11(12), 1768-1776 (2012-09-12)
Phosphorylase kinase (PhK), a 1.3 MDa enzyme complex that regulates glycogenolysis, is composed of four copies each of four distinct subunits (α, β, γ, and δ). The catalytic protein kinase subunit within this complex is γ, and its activity is
Monica Balsera et al.
Planta, 237(2), 619-635 (2012-12-12)
Uncovered in studies on photosynthesis 35 years ago, redox regulation has been extended to all types of living cells. We understand a great deal about the occurrence, function, and mechanism of action of this mode of regulation, but we know little
Antisense suppression of the small chloroplast protein CP12 in tobacco: a transcriptional viewpoint.
Thomas P Howard et al.
Plant signaling & behavior, 6(12), 2026-2030 (2011-11-25)
The chloroplast protein CP12 forms a multi-enzyme complex with the Calvin-Benson cycle enzymes phosphoribulokinase (PRK) and NADP-glyceraldehyde-3-phosphate dehydrogenase (GAPDH). PRK and GAPDH are inactivated when present in this complex, a process shown in vitro to be dependent upon oxidized CP12.
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